2026-06-14T10:33:44Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/68302025-04-08T10:22:37Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Martínez-Sernández, Victoria author Muiño, Laura author Perteguer-Prieto, Maria Jesus author Garate, Teresa author Mezo, Mercedes author González-Warleta, Marta author Muro, Antonio author Correia da Costa, José Manuel author Romarís, Fernanda author Ubeira, Florencio M author 2011-11-08 BACKGROUND: Human fasciolosis is a re-emerging disease worldwide and is caused by species of the genus Fasciola (F. hepatica and F. gigantica). Human fasciolosis can be diagnosed by classical coprological techniques, such as the Kato-Katz test, to reveal parasite eggs in faeces. However, although 100% specific, these methods are generally not adequate for detection of acute infections, ectopic infections, or infections with low number of parasites. In such cases immunological methods may be a good alternative and are recommended for use in major hospitals where trained personnel are available, although they are not usually implemented for individual testing. METHODOLOGY/PRINCIPAL FINDINGS: We have developed a new lateral flow test (SeroFluke) for the serodiagnosis of human fasciolosis. The new test was constructed with a recombinant cathepsin L1 from F. hepatica, and uses protein A and mAb MM3 as detector reagents in the test and control lines, respectively. In comparison with an ELISA test (MM3-SERO) the SeroFluke test showed maximal specificity and sensitivity and can be used with serum or whole blood samples. CONCLUSIONS/SIGNIFICANCE: The new test can be used in major hospitals in hypoendemic countries as well as in endemic/hyperendemic regions where point-of-care testing is required. PLoS Negl Trop Dis. 2011 Nov;5(11):e1376. 10.1371/journal.pntd.0001376 1935-2735 1935-2735 PLoS neglected tropical diseases 22087343 http://hdl.handle.net/20.500.12105/6830 Development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis