2026-07-24T06:28:59Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/68212024-11-29T14:34:48Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597

00925njm 22002777a 4500 dc Garcia-Carpizo, Veronica author Ruiz-Llorente, Sergio author Sarmentero, Jacinto author Graña Castro, Osvaldo author Pisano, David G author Barrero, Maria Jose author 2018 BACKGROUND: The reported antitumor activity of the BET family bromodomain inhibitors has prompted the development of inhibitors against other bromodomains. However, the human genome encodes more than 60 different bromodomains and most of them remain unexplored. RESULTS: We report that the bromodomains of the histone acetyltransferases CREBBP/EP300 are critical to sustain the proliferation of human leukemia and lymphoma cell lines. EP300 is very abundant at super-enhancers in K562 and is coincident with sites of GATA1 and MYC occupancy. In accordance, CREBBP/EP300 bromodomain inhibitors interfere with GATA1- and MYC-driven transcription, causing the accumulation of cells in the G0/G1 phase of the cell cycle. The CREBBP/CBP30 bromodomain inhibitor CBP30 displaces CREBBP and EP300 from GATA1 and MYC binding sites at enhancers, resulting in a decrease in the levels of histone acetylation at these regulatory regions and consequently reduced gene expression of critical genes controlled by these transcription factors. CONCLUSIONS: Our data shows that inhibition of CREBBP/EP300 bromodomains can interfere with oncogene-driven transcriptional programs in cancer cells and consequently hold therapeutic potential. Epigenetics Chromatin. 2018;11(1):30. 10.1186/s13072-018-0197-x 1756-8935 1756-8935 Epigenetics & chromatin 29884215 http://hdl.handle.net/20.500.12105/6821 Bromodomains Cancer Epigenetics Proliferation CREBBP/EP300 bromodomains are critical to sustain the GATA1/MYC regulatory axis in proliferation