2026-05-21T23:11:54Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/67102025-05-27T13:35:02Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Alcolea, Pedro J
author
Alonso, Ana
author
Moreno-Izquierdo, Miguel A
author
Degayón, María A
author
Moreno-Iruela, Inmaculada
author
Larraga, Vicente
funder
Ministerio de Economía y Competitividad (España)
2018-11-23T12:03:31Z
2018-11-23T12:03:31Z
2016-03-09
PLoS One. 2016 Mar 9;11(3):e0150172.
10.1371/journal.pone.0150172
1932-6203
1932-6203
PloS one
26959417
http://hdl.handle.net/20.500.12105/6710
Leishmania infantum is one of the species responsible for visceral leishmaniasis. This species is distributed basically in the Mediterranean basin. A recent outbreak in humans has been reported in Spain. Axenic cultures are performed for most procedures with Leishmania spp. promastigotes. This model is stable and reproducible and mimics the conditions of the gut of the sand fly host, which is the natural environment of promastigote development. Culture media are undefined because they contain mammalian serum, which is a rich source of complex lipids and proteins. Serum deprivation slows down the growth kinetics and therefore, yield in biomass. In fact, we have confirmed that the growth rate decreases, as well as infectivity. Ploidy is also affected. Regarding the transcriptome, a high-throughput approach has revealed a low differential expression rate but important differentially regulated genes. The most remarkable profiles are: up-regulation of the GINS Psf3, the fatty acyl-CoA synthase (FAS1), the glyoxylase I (GLO1), the hydrophilic surface protein B (HASPB), the methylmalonyl-CoA epimerase (MMCE) and an amastin gene; and down-regulation of the gPEPCK and the arginase. Implications for metabolic adaptations, differentiation and infectivity are discussed herein.
eng
Serum Removal from Culture Induces Growth Arrest, Ploidy Alteration, Decrease in Infectivity and Differential Expression of Crucial Genes in Leishmania infantum Promastigotes
research article
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URL
https://repisalud.isciii.es/bitstreams/687a0b09-4aca-491c-a5c5-7b8578dd79a2/download
File
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application/pdf
SerumRemovalFromCulture_2016.pdf
URL
https://repisalud.isciii.es/bitstreams/75161608-cd57-47f1-ab5d-b38b6f3d4018/download
File
MD5
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text/plain
SerumRemovalFromCulture_2016.pdf.txt