2026-06-14T03:38:28Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/66892024-09-27T09:45:24Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc van Dinther, Dieke author Veninga, Henrike author Iborra, Salvador author Borg, Ellen G. F. author Hoogterp, Leoni author Olesek, Katarzyna author Beijer, Marieke R. author Schetters, Sjoerd T. T. author Kalay, Hakan author Garcia-Vallejo, Juan J. author Franken, Kees L. author Cham, Lamin B. author Lang, Karl S. author van Kooyk, Yvette author Sancho, David author Crocker, Paul R. author den Haan, Joke M. M. author 2018 Splenic CD169(+) macrophages are located in the marginal zone to efficiently capture blood-borne pathogens. Here, we investigate the requirements for the induction of CD8(+) T cell responses by antigens (Ags) bound by CD169(+) macrophages. Upon Ag targeting to CD169(+) macrophages, we show that BATF3-dependent CD8 alpha(+) dendritic cells (DCs) are crucial for DNGR-1-mediated cross-priming of CD8(+) T cell responses. In addition, we demonstrate that CD169, a sialic acid binding lectin involved in cell-cell contact, preferentially binds to CD8 alpha(+) DCs and that Ag transfer to CD8 alpha(+) DCs and subsequent T cell activation is dependent on the sialic acid-binding capacity of CD169. Finally, functional CD169 mediates optimal CD8(+) T cell responses to modified vaccinia Ankara virus infection. Together, these data indicate that the collaboration of CD169(+) macrophages and CD8 alpha(+) DCs for the initiation of effective CD8(+) T cell responses is facilitated by binding of CD169 to sialic acid containing ligands on CD8 alpha(+) DCs. Cell Rep. 2018; 22(6):1484-1495 10.1016/j.celrep.2018.01.021 2211-1247 Cell Reports 29425504 http://hdl.handle.net/20.500.12105/6689 Functional CD169 on Macrophages Mediates Interaction with Dendritic Cells for CD8(+) T Cell Cross-Priming