2026-06-14T03:33:52Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/66782025-05-07T12:26:28Zcom_20.500.12105_19604com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19605col_20.500.12105_16938col_20.500.12105_16988
Repisalud
author
Garde, Esther
author
Ramirez, Laura
author
Corvo, Laura
author
Solana, Jose C.
author
Elena Martin, M.
author
González, Victor M
author
Gomez-Nieto, Carlos
author
Barral, Aldina
author
Barral-Netto, Manoel
author
Requena, Jose M
author
Iborra, Salvador
author
Soto, Manuel
funder
Ministerio de Ciencia e Innovación (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Ministerio de Economía y Competitividad (España)
funder
Fundación Ramón Areces
funder
Banco Santander
2018-11-22T08:10:52Z
2018-11-22T08:10:52Z
2018
Front Cell Infect Microbiol. 2018; 8:112
10.3389/fcimb.2018.00112
2235-2988
Frontiers in Cellular and Infection Microbiology
29675401
http://hdl.handle.net/20.500.12105/6678
Different members of intracellular protein families are recognized by the immune system of the vertebrate host infected by parasites of the genus Leishmania. Here, we have analyzed the antigenic and immunogenic properties of the Leishmania eIF2 and eIF2B translation initiation factors. An in silico search in Leishmania infantum sequence databases allowed the identification of the genes encoding the alpha, beta, and gamma subunits and the alpha, beta, and delta subunits of the putative Leishmania orthologs of the eukaryotic initiation factors F2 (LieIF2) or F2B (LieIF2B), respectively. The antigenicity of these factors was analyzed by ELISA using recombinant versions of the different subunits. Antibodies against the different LieIF2 and LieIF2B subunits were found in the sera from human and canine visceral leishmaniasis patients, and also in the sera from hamsters experimentally infected with L. infantum. In L. infantum (BALB/c) and Leishmania major (BALB/c or C57BL/6) challenged mice, a moderate humoral response against these protein factors was detected. Remarkably, these proteins elicited an IL-10 production by splenocytes derived from infected mice independently of the Leishmania species employed for experimental challenge. When DNA vaccines based on the expression of the LieIF2 or LieIF2B subunit encoding genes were administered inmice, an antigen-specific secretion of IFN-gamma and IL-10 cytokines was observed. Furthermore, a partial protection against murine CL development due to L. major infection was generated in the vaccinated mice. Also, in this work we show that the LieIF2 alpha subunit and the LieIF2B beta and delta subunits have the capacity to stimulate IL-10 secretion by spleen cells from naive mice. B-lymphocytes were identified as the major producers of this anti-inflammatory cytokine. Taking into account the data found in this study, it may be hypothesized that these proteins act as virulence factors implicated in the induction of humoral responses as well as in the production of the down-regulatory IL-10 cytokine, favoring a pathological outcome. Therefore, these proteins might be considered markers of disease.
eng
Leishmania
Antigens
Interleukin-10
Visceral leishmaniasis
Translation initiation factors
Experimental murine models
Vaccines
Analysis of the Antigenic and Prophylactic Properties of the Leishmania Translation Initiation Factors eIF2 and eIF2B in Natural and Experimental Leishmaniasis
research article
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AnalysisAntigenicProphylactic_2018.pdf
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AnalysisAntigenicProphylactic_2018_sm1.pdf
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AnalysisAntigenicProphylactic_2018.pdf.txt
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AnalysisAntigenicProphylactic_2018_sm1.pdf.txt