2026-06-14T12:12:36Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/66772024-09-27T09:34:54Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607
Repisalud
author
Walter, Wencke
author
Alonso-Herranz, Laura
author
Trappetti, Verdiana
author
Crespo, Isaac
author
Ibberson, Mark
author
Cedenilla, Marta
author
Karaszewska, Anna
author
Nunez, Vanessa
author
Xenarios, Ioannis
author
Arroyo, Alicia G
author
Sanchez-Cabo, Fatima
author
Ricote, Mercedes
funder
Ministerio de Economía y Competitividad (España)
funder
Fundación La Marató TV3
funder
Unión Europea. Comisión Europea
funder
Fundación La Caixa
funder
Ministerio de Economía, Industria y Competitividad (España)
funder
Fundación ProCNIC
2018-11-22T08:10:52Z
2018-11-22T08:10:52Z
2018
Cell Rep. 2018; 23(2):622-636
10.1016/j.celrep.2018.03.029
2211-1247
Cell Reports
29642017
http://hdl.handle.net/20.500.12105/6677
Macrophage plasticity has been studied in vitro, but transcriptional regulation upon injury is poorly understood. We generated a valuable dataset that captures transcriptional changes in the healthy heart and after myocardial injury, revealing a dynamic transcriptional landscape of macrophage activation. Partial deconvolution suggested that post-injury macrophages exhibit overlapping activation of pro-inflammatory and anti-inflammatory programs rather than aligning to canonical M1/M2 programs. Furthermore, simulated dynamics and experimental validation of a regulatory core of the underlying gene-regulatory network revealed a negative-feedback loop that limits initial inflammation via hypoxia-mediated upregulation of ll10. Our results also highlight the prominence of post-transcriptional regulation (miRNAs, mRNA decay, and lincRNAs) in attenuating the myocardial injury-induced inflammatory response. We also identified a cardiac-macrophage-specific gene signature (e.g., Egfr and Lifr) and time-specific markers for macrophage populations (e.g., Lyve1, Cd40, and Mrc1). Altogether, these data provide a core resource for deciphering the transcriptional network in cardiac macrophages in vivo.
eng
Deciphering the Dynamic Transcriptional and Post-transcriptional Networks of Macrophages in the Healthy Heart and after Myocardial Injury
journal article
URL
https://repisalud.isciii.es/bitstreams/c452d663-82cf-4bef-8969-59ba5c6e51ca/download
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application/pdf
DecipheringTheDynamicTranscriptional_2018.pdf
URL
https://repisalud.isciii.es/bitstreams/3b4f45b9-5e4d-4dca-b1c4-78d141183892/download
File
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text/plain
DecipheringTheDynamicTranscriptional_2018.pdf.txt