2026-05-17T01:43:55Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/66752024-09-27T08:58:55Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Blazquez, Rebeca
author
Sánchez-Margallo, Francisco Miguel
author
Alvarez, Veronica
author
Matilla, Elvira
author
Hernandez, Nuria
author
Marinaro, Federica
author
Gomez-Serrano, Maria
author
Jorge, Inmaculada
author
Casado, Javier G.
author
Macias-Garcia, Beatriz
funder
Centro de Investigación Biomedica en Red - CIBER
funder
Government of Extremadura (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Instituto de Salud Carlos III
funder
Ministerio de Economía, Industria y Competitividad (España)
2018-11-22T08:10:51Z
2018-11-22T08:10:51Z
2018
PLoS One. 2018; 13(4):e0196080
10.1371/journal.pone.0196080
1932-6203
PLoS ONE
29684038
http://hdl.handle.net/20.500.12105/6675
Endometrial Mesenchymal Stromal Cells (endMSCs) are multipotent cells with immunomodulatory and pro-regenerative activity which is mainly mediated by a paracrine effect. The exosomes released by MSCs have become a promising therapeutic tool for the treatment of immune-mediated diseases. More specifically, extracellular vesicles derived from endMSCs (EV-endMSCs) have demonstrated a cardioprotective effect through the release of anti-apoptotic and pro-angiogenic factors. Here we hypothesize that EV-endMSCs may be used as a co-adjuvant to improve in vitro fertilization outcomes and embryo quality. Firstly, endMSCs and EV-endMSCs were isolated and phenotypically characterized for in vitro assays. Then, in vitro studies were performed on murine embryos co-cultured with EV-endMSCs at different concentrations. Our results firstly demonstrated a significant increase on the total blastomere count of expanded murine blastocysts. Moreover, EV-endMSCs triggered the release of pro-angiogenic molecules from embryos demonstrating an EV-endMSCs concentration-dependent increase of VEGF and PDGF-AA. The release of VEGF and PDGF-AA by the embryos may indicate that the beneficial effect of EV-endMSCs could be mediating not only an increase in the blastocyst's total cell number, but also may promote endometrial angiogenesis, vascularization, differentiation and tissue remodeling. In summary, these results could be relevant for assisted reproduction being the first report describing the beneficial effect of human EV-endMSCs on embryo development.
eng
ENDOTHELIAL GROWTH-FACTOR
MESENCHYMAL STEM-CELLS
IN-VITRO
CULTURE-MEDIUM
MOUSE EMBRYO
AVIAN EMBRYO
IMPLANTATION
EXOSOMES
ANGIOGENESIS
PDGF
Murine embryos exposed to human endometrial MSCs-derived extracellular vesicles exhibit higher VEGF/PDGF AA release, increased blastomere count and hatching rates
journal article
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MurineEmbryosExposedHuman_2018.pdf
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image/tiff
MurineEmbryosExposedHuman_2018_S1Fig.tif
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MurineEmbryosExposedHuman_2018.pdf.txt