2026-05-17T01:43:54Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/66752024-09-27T08:58:55Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Blazquez, Rebeca author Sánchez-Margallo, Francisco Miguel author Alvarez, Veronica author Matilla, Elvira author Hernandez, Nuria author Marinaro, Federica author Gomez-Serrano, Maria author Jorge, Inmaculada author Casado, Javier G. author Macias-Garcia, Beatriz author 2018 Endometrial Mesenchymal Stromal Cells (endMSCs) are multipotent cells with immunomodulatory and pro-regenerative activity which is mainly mediated by a paracrine effect. The exosomes released by MSCs have become a promising therapeutic tool for the treatment of immune-mediated diseases. More specifically, extracellular vesicles derived from endMSCs (EV-endMSCs) have demonstrated a cardioprotective effect through the release of anti-apoptotic and pro-angiogenic factors. Here we hypothesize that EV-endMSCs may be used as a co-adjuvant to improve in vitro fertilization outcomes and embryo quality. Firstly, endMSCs and EV-endMSCs were isolated and phenotypically characterized for in vitro assays. Then, in vitro studies were performed on murine embryos co-cultured with EV-endMSCs at different concentrations. Our results firstly demonstrated a significant increase on the total blastomere count of expanded murine blastocysts. Moreover, EV-endMSCs triggered the release of pro-angiogenic molecules from embryos demonstrating an EV-endMSCs concentration-dependent increase of VEGF and PDGF-AA. The release of VEGF and PDGF-AA by the embryos may indicate that the beneficial effect of EV-endMSCs could be mediating not only an increase in the blastocyst's total cell number, but also may promote endometrial angiogenesis, vascularization, differentiation and tissue remodeling. In summary, these results could be relevant for assisted reproduction being the first report describing the beneficial effect of human EV-endMSCs on embryo development. PLoS One. 2018; 13(4):e0196080 10.1371/journal.pone.0196080 1932-6203 PLoS ONE 29684038 http://hdl.handle.net/20.500.12105/6675 ENDOTHELIAL GROWTH-FACTOR MESENCHYMAL STEM-CELLS IN-VITRO CULTURE-MEDIUM MOUSE EMBRYO AVIAN EMBRYO IMPLANTATION EXOSOMES ANGIOGENESIS PDGF Murine embryos exposed to human endometrial MSCs-derived extracellular vesicles exhibit higher VEGF/PDGF AA release, increased blastomere count and hatching rates