2026-04-26T23:36:34Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/66522024-09-27T08:57:28Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607
Repisalud
author
Groult, Hugo
author
Ruiz-Cabello, Jesus
author
Lechuga-Vieco, Ana V.
author
Mateo, Jesus
author
Benito, Marina
author
Bilbao, Izaskun
author
Martínez-Alcázar, María Paz
author
Lopez, Juan Antonio
author
Vazquez, Jesus
author
Herranz, Fernando
funder
Unión Europea. Comisión Europea
funder
Fundación La Marató TV3
funder
Ministerio de Economía y Competitividad (España)
funder
Comunidad de Madrid (España)
2018-11-21T08:50:02Z
2018-11-21T08:50:02Z
2014-12-08
Chemistry, 2014. 20(50): p. 16662-71
09476539
http://hdl.handle.net/20.500.12105/6652
25319949
10.1002/chem.201404221
1521-3765
Chemistry (Weinheim an der Bergstrasse, Germany)
We report the synthesis of micellar phosphatidylcholine-coated superparamagnetic iron oxide nanoparticles as a new long circulation contrast agents for magnetic resonance imaging. Oleic acid-coated Fe3 O4 nanoparticles were first prepared through thermal degradation and then encapsulated into small clusters with a phosphatidylcholine coating to obtain hydrophilic nanomicelles. A thorough characterization confirmed the chemical nature of the coating and the excellent colloidal stability of these nanomicelles in aqueous media. Magnetization and relaxivity properties proved their suitability as magnetic resonance imaging (MRI) contrast agent and in vitro cell viability data showed low toxicity. Vascular lifetime and elimination kinetics in the liver were assessed by blood relaxometry and by in vivo MRI in rats and compared with "control" particles prepared with a polyethylene glycol derivative. These micellar particles had a lifetime in blood of more than 10 h, much longer than the control nanoparticles (≈2 h), which is remarkable considering that the coating molecule is a small biocompatible zwitterionic phospholipid. The protein corona was characterized after incubation with rat serum at different times by high-throughput proteomics, showing a higher proportion of bound apolipoproteins and other dysopsonins for the phosphatidylcholine particles. The antibiofouling properties of this corona and its resistance to the adsorption of proteins corroborate the observed enhanced stability and prolonged systemic circulation.
eng
iron
magnetic properties
micelles
nanoparticles
proteins
Phosphatidylcholine-coated iron oxide nanomicelles for in vivo prolonged circulation time with an antibiofouling protein corona
journal article
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