2026-06-30T02:00:52Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/66432024-09-27T08:03:07Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607

00925njm 22002777a 4500 dc Isern, Joan author Martín-Antonio, Beatriz author Ghazanfari, Roshanak author Martin, Ana M. author Lopez, Juan Antonio author Del Toro, Raquel author Sanchez-Aguilera, Abel author Arranz, Lorena author Martin-Perez, Daniel author Suárez-Lledó, María author Marín, Pedro author Van Pel, Melissa author Fibbe, Willem E author Vazquez, Jesus author Scheding, Stefan author Urbano-Ispizúa, Álvaro author Mendez-Ferrer, Simon author 2013-05-30 Strategies for expanding hematopoietic stem cells (HSCs) include coculture with cells that recapitulate their natural microenvironment, such as bone marrow stromal stem/progenitor cells (BMSCs). Plastic-adherent BMSCs may be insufficient to preserve primitive HSCs. Here, we describe a method of isolating and culturing human BMSCs as nonadherent mesenchymal spheres. Human mesenspheres were derived from CD45- CD31- CD71- CD146+ CD105+ nestin+ cells but could also be simply grown from fetal and adult BM CD45--enriched cells. Human mesenspheres robustly differentiated into mesenchymal lineages. In culture conditions where they displayed a relatively undifferentiated phenotype, with decreased adherence to plastic and increased self-renewal, they promoted enhanced expansion of cord blood CD34+ cells through secreted soluble factors. Expanded HSCs were serially transplantable in immunodeficient mice and significantly increased long-term human hematopoietic engraftment. These results pave the way for culture techniques that preserve the self-renewal of human BMSCs and their ability to support functional HSCs. Cell Rep. 2013 May 30;3(5):1714-24 10.1016/j.celrep.2013.03.041 2211-1247 22111247 Cell reports 23623496 http://hdl.handle.net/20.500.12105/6643 Self-renewing human bone marrow mesenspheres promote hematopoietic stem cell expansion