2026-08-05T11:38:46Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/66372025-04-28T07:34:24Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Rodriguez, Ariel author Falcon, Ana author Cuevas, Maria Teresa author Pozo Sanchez, Francisco author Guerra, Susana author Garcia-Barreno, Blanca author Martínez-Orellana, Pamela author Perez-Breña, Pilar author Montoya, Maria author Melero, Jose Antonio author Pizarro, Manuel author Ortin, Juan author Casas Flecha, Inmaculada author Nieto, Amelia author 2013-01-10 Pandemic 2009 H1N1 (pH1N1) influenza viruses caused mild symptoms in most infected patients. However, a greater rate of severe disease was observed in healthy young adults and children without co-morbid conditions. Here we tested whether influenza strains displaying differential virulence could be present among circulating pH1N1 viruses. The biological properties and the genotype of viruses isolated from a patient showing mild disease (M) or from a fatal case (F), both without known co-morbid conditions were compared in vitro and in vivo. The F virus presented faster growth kinetics and stronger induction of cytokines than M virus in human alveolar lung epithelial cells. In the murine model in vivo, the F virus showed a stronger morbidity and mortality than M virus. Remarkably, a higher proportion of mice presenting infectious virus in the hearts, was found in F virus-infected animals. Altogether, the data indicate that strains of pH1N1 virus with enhanced pathogenicity circulated during the 2009 pandemic. In addition, examination of chemokine receptor 5 (CCR5) genotype, recently reported as involved in severe influenza virus disease, revealed that the F virus-infected patient was homozygous for the deleted form of CCR5 receptor (CCR5Δ32). PLoS One. 2013;8(1):e53515 10.1371/journal.pone.0053515 1932-6203 1932-6203 PloS one 23326447 http://hdl.handle.net/20.500.12105/6637 Characterization in vitro and in vivo of a pandemic H1N1 influenza virus from a fatal case