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00925njm 22002777a 4500 dc Gonzalez, Luis Miguel author Cuevas, Laureano author Perez-Pastrana, Esperanza author Santa-María, Ysmael author Benito, Agustin author 2009-10-09 To identity immunodiagnostic antigen genes, a Plasmodium falciparum (Dd2 clone) expression library was screened using human immune sera. The ring-infected erythrocyte surface antigen (RESA) was isolated: this antigen of the resistant clone presents repeat tandem sequences like the 3D7 clone, albeit in different numbers. RESA has been studied as a marker of genetic diversity, with different sizes being observed in different isolates and clones of Plasmodium falciparum. The native protein was localised in cultures by western-blot and immuno-transmission electron microscopy. The antigenicity of RESA was evaluated by ELISA, using the carboxy-terminal repeat region as antigen. The assay's sensitivity and specificity were 78.2 and 94% respectively. Mol Biol Rep. 2010;37(6):2893-902. 10.1007/s11033-009-9849-z 1573-4978 0301-4851 Molecular biology reports 19816792 http://hdl.handle.net/20.500.12105/6634 Plasmodium falciparum Malaria ELISA Expression library Electron microscopy Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum