2026-06-14T03:29:57Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/65812024-09-27T10:11:48Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19607
Repisalud
author
Erami, Roghayeh Sadeghi
author
Ovejero, Karina
author
Meghdadi, Soraia
author
Filice, Marco
author
Amirnasr, Mehdi
author
Rodriguez-Dieguez, Antonio
author
De La Orden, Maria Ulagares
author
Gomez-Ruiz, Santiago
funder
Ministerio de Economía y Competitividad (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
King Juan Carlos University (España)
funder
Isfahan University of Technology (Irán)
funder
Iran National Science Foundation
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Fundación ProCNIC
funder
Complutense University of Madrid (España)
funder
Comunidad de Madrid (España)
2018-11-05T11:58:22Z
2018-11-05T11:58:22Z
2018
Nanomaterials (Basel). 2018; 8(6):434
10.3390/nano8060434
2079-4991
Nanomaterials
29903996
http://hdl.handle.net/20.500.12105/6581
Functionalized magnetite nanoparticles (FMNPs) and functionalized mesoporous silica nanoparticles (FMSNs) were synthesized by the conjugation of magnetite and mesoporous silica with the small and fluorogenic benzothiazole ligand, that is, 2(2-hydroxyphenyl)benzothiazole (hpbtz). The synthesized fluorescent nanoparticles were characterized by FTIR, XRD, XRF, C-13 CP MAS NMR, BET, and TEM. The photophysical behavior of FMNPs and FMSNs in ethanol was studied using fluorescence spectroscopy. The modification of magnetite and silica scaffolds with the highly fluorescent benzothiazole ligand enabled the nanoparticles to be used as selective and sensitive optical probes for zinc ion detection. Moreover, the presence of hpbtz in FMNPs and FMSNs induced efficient cell viability and zinc ion uptake, with desirable signaling in the normal human kidney epithelial (Hek293) cell line. The significant viability of FMNPs and FMSNs (80\% and 92\%, respectively) indicates a potential applicability of these nanoparticles as in vitro imaging agents. The calculated limit of detections (LODs) were found to be 2.53 x 10(-6) and 2.55 x 10(-6) M for Fe3O4-H@hpbtz and MSN-Et3N-IPTMS-hpbtz-f1, respectively. FMSNs showed more pronounced zinc signaling relative to FMNPs, as a result of the more efficient penetration into the cells.
eng
Nanomaterials
Mesoporous silica
Magnetite
Live cell imaging
Zn2+ detection
Zn2+ sensors
COMBINED IONOPHORE/FLUOROPHORE MOIETIES
FLUORESCENT-PROBE
OXIDATIVE STRESS
AQUEOUS-SOLUTION
LIVING CELLS
ZN2+ ION
NANOPARTICLES
COMPLEXES
SENSOR
BENZOTHIAZOLE
Applications of Nanomaterials Based on Magnetite and Mesoporous Silica on the Selective Detection of Zinc Ion in Live Cell Imaging
journal article
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ApplicationsNanomaterialsBasedMagnetite_2018.pdf
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ApplicationsNanomaterialsBasedMagnetite_2018_SUPPL.pdf
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ApplicationsNanomaterialsBasedMagnetite_2018.pdf.txt
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ApplicationsNanomaterialsBasedMagnetite_2018_SUPPL.pdf.txt