2026-06-14T03:32:27Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/65812024-09-27T10:11:48Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19607

00925njm 22002777a 4500 dc Erami, Roghayeh Sadeghi author Ovejero, Karina author Meghdadi, Soraia author Filice, Marco author Amirnasr, Mehdi author Rodriguez-Dieguez, Antonio author De La Orden, Maria Ulagares author Gomez-Ruiz, Santiago author 2018 Functionalized magnetite nanoparticles (FMNPs) and functionalized mesoporous silica nanoparticles (FMSNs) were synthesized by the conjugation of magnetite and mesoporous silica with the small and fluorogenic benzothiazole ligand, that is, 2(2-hydroxyphenyl)benzothiazole (hpbtz). The synthesized fluorescent nanoparticles were characterized by FTIR, XRD, XRF, C-13 CP MAS NMR, BET, and TEM. The photophysical behavior of FMNPs and FMSNs in ethanol was studied using fluorescence spectroscopy. The modification of magnetite and silica scaffolds with the highly fluorescent benzothiazole ligand enabled the nanoparticles to be used as selective and sensitive optical probes for zinc ion detection. Moreover, the presence of hpbtz in FMNPs and FMSNs induced efficient cell viability and zinc ion uptake, with desirable signaling in the normal human kidney epithelial (Hek293) cell line. The significant viability of FMNPs and FMSNs (80\% and 92\%, respectively) indicates a potential applicability of these nanoparticles as in vitro imaging agents. The calculated limit of detections (LODs) were found to be 2.53 x 10(-6) and 2.55 x 10(-6) M for Fe3O4-H@hpbtz and MSN-Et3N-IPTMS-hpbtz-f1, respectively. FMSNs showed more pronounced zinc signaling relative to FMNPs, as a result of the more efficient penetration into the cells. Nanomaterials (Basel). 2018; 8(6):434 10.3390/nano8060434 2079-4991 Nanomaterials 29903996 http://hdl.handle.net/20.500.12105/6581 Nanomaterials Mesoporous silica Magnetite Live cell imaging Zn2+ detection Zn2+ sensors COMBINED IONOPHORE/FLUOROPHORE MOIETIES FLUORESCENT-PROBE OXIDATIVE STRESS AQUEOUS-SOLUTION LIVING CELLS ZN2+ ION NANOPARTICLES COMPLEXES SENSOR BENZOTHIAZOLE Applications of Nanomaterials Based on Magnetite and Mesoporous Silica on the Selective Detection of Zinc Ion in Live Cell Imaging