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oai:repisalud.isciii.es:20.500.12105/65792024-09-27T10:15:24Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19607

00925njm 22002777a 4500 dc Vinambres, Mario author Filice, Marco author Marciello, Marzia author 2018 The immobilization of biocatalysts on magnetic nanomaterial surface is a very attractive alternative to achieve enzyme nanoderivatives with highly improved properties. The combination between the careful tailoring of nanocarrier surfaces and the site-specific chemical modification of biomacromolecules is a crucial parameter to finely modulate the catalytic behavior of the biocatalyst. In this work, a useful strategy to immobilize chemically aminated lipase B from Candida antarctica on magnetic iron oxide nanoparticles (IONPs) by covalent multipoint attachment or hydrophobic physical adsorption upon previous tailored engineering of nanocarriers with poly-carboxylic groups (citric acid or succinic anhydride, CALB(EDA)@CA-NPs and CALB(EDA)@SA-NPs respectively) or hydrophobic layer (oleic acid, CALB(EDA)@OA-NPs) is described. After full characterization, the nanocatalysts have been assessed in the enantioselective kinetic resolution of racemic methyl mandelate. Depending on the immobilization strategy, each enzymatic nanoderivative permitted to selectively improve a specific property of the biocatalyst. In general, all the immobilization protocols permitted loading from good to high lipase amount (149 < immobilized lipase < 234 mg/g(Fe)). The hydrophobic CALB(EDA)@OA-NPs was the most active nanocatalyst, whereas the covalent CALB(EDA)@CA-NPs and CALB(EDA)@SA-NPs were revealed to be the most thermostable and also the most enantioselective ones in the kinetic resolution reaction (almost 90\% ee R-enantiomer). A strategy to maintain all these properties in long-time storage (up to 1 month) by freeze-drying was also optimized. Therefore, the nanocarrier surface engineering is demonstrated to be a key-parameter in the design and preparation of lipase libraries with enhanced catalytic properties. Polymers. 2018; 10(6):615 10.3390/polym10060615 2073-4360 Polymers http://hdl.handle.net/20.500.12105/6579 Colloid surface engineering Magnetic iron oxide nanoparticles Oriented immobilization Lipase Catalysis Nanotechnology Nanobiocatalyst Freeze-drying GAMMA-FE2O3 NANOPARTICLES BIOMEDICAL APPLICATIONS ENZYME IMMOBILIZATION CHEMICAL-MODIFICATION HYDROPHOBIC SUPPORTS SOLID-PHASE STRATEGIES STABILIZATION BIOCATALYSTS HYPERTHERMIA Modulation of the Catalytic Properties of Lipase B from Candida antarctica by Immobilization on Tailor-Made Magnetic Iron Oxide Nanoparticles: The Key Role of Nanocarrier Surface Engineering