2026-07-24T06:25:06Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/65752024-09-27T10:04:09Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19607
Repisalud
author
Callejo, Maria
author
Mondejar-Parreno, Gema
author
Barreira, Bianca
author
Izquierdo-Garcia, Jose L.
author
Morales-Cano, Daniel
author
Esquivel-Ruiz, Sergio
author
Moreno, Laura
author
Cogolludo, Angel
author
Duarte, Juan
author
Perez-Vizcaino, Francisco
funder
Ministerio de Economía y Competitividad (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Complutense University of Madrid (España)
funder
Fundación Contra la Hipertensión Pulmonar
funder
Ministerio de Educación (España)
funder
Unión Europea. Comisión Europea
funder
Agencia Estatal de Investigación (España)
funder
Fundación ProCNIC
funder
Comunidad de Madrid (España)
2018-11-05T11:58:21Z
2018-11-05T11:58:21Z
2018
Sci Rep. 2018; 8(1):9681
10.1038/s41598-018-27682-w
2045-2322
Scientific Reports
29946072
http://hdl.handle.net/20.500.12105/6575
We have analysed whether pulmonary arterial hypertension (PAH) alters the rat faecal microbiota. Wistar rats were injected with the VEGF receptor antagonist SU5416 (20 mg/kg s.c.) and followed for 2 weeks kept in hypoxia (10\% O-2, PAH) or injected with vehicle and kept in normoxia (controls). Faecal samples were obtained and microbiome composition was determined by 165 rRNA gene sequencing and bioinformatic analysis. No effect of PAH on the global microbiome was found (alpha- or beta-diversity). However, PAH-exposed rats showed gut dysbiosis as indicated by a taxonomy-based analysis. Specifically, PAH rats had a three-fold increase in Firmicutes-to-Bacteroidetes ratio. Within the Firmicutes phylum, there were no large changes in the relative abundance of the bacterial families in PAH. Among Bacteroidetes, all families were less abundant in PAH. A clear separation was observed between the control and PAH clusters based on short chain fatty acid producing bacterial genera. Moreover, acetate was reduced in the serum of PAH rats. In conclusion, faecal microbiota composition is altered as a result of PAH. This misbalanced bacterial ecosystem might in turn play a pathophysiological role in PAH by altering the immunologic, hormonal and metabolic homeostasis.
eng
SLEEP-APNEA
DISEASE
DYSBIOSIS
IMMUNITY
INFLAMMATION
INFECTION
BACTERIA
LUNG
CARCINOGENESIS
INHIBITION
Pulmonary Arterial Hypertension Affects the Rat Gut Microbiome
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PulmonaryArterialHypertensionAffects_2018.pdf.txt