2026-07-24T06:25:05Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/65752024-09-27T10:04:09Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19607

00925njm 22002777a 4500 dc Callejo, Maria author Mondejar-Parreno, Gema author Barreira, Bianca author Izquierdo-Garcia, Jose L. author Morales-Cano, Daniel author Esquivel-Ruiz, Sergio author Moreno, Laura author Cogolludo, Angel author Duarte, Juan author Perez-Vizcaino, Francisco author 2018 We have analysed whether pulmonary arterial hypertension (PAH) alters the rat faecal microbiota. Wistar rats were injected with the VEGF receptor antagonist SU5416 (20 mg/kg s.c.) and followed for 2 weeks kept in hypoxia (10\% O-2, PAH) or injected with vehicle and kept in normoxia (controls). Faecal samples were obtained and microbiome composition was determined by 165 rRNA gene sequencing and bioinformatic analysis. No effect of PAH on the global microbiome was found (alpha- or beta-diversity). However, PAH-exposed rats showed gut dysbiosis as indicated by a taxonomy-based analysis. Specifically, PAH rats had a three-fold increase in Firmicutes-to-Bacteroidetes ratio. Within the Firmicutes phylum, there were no large changes in the relative abundance of the bacterial families in PAH. Among Bacteroidetes, all families were less abundant in PAH. A clear separation was observed between the control and PAH clusters based on short chain fatty acid producing bacterial genera. Moreover, acetate was reduced in the serum of PAH rats. In conclusion, faecal microbiota composition is altered as a result of PAH. This misbalanced bacterial ecosystem might in turn play a pathophysiological role in PAH by altering the immunologic, hormonal and metabolic homeostasis. Sci Rep. 2018; 8(1):9681 10.1038/s41598-018-27682-w 2045-2322 Scientific Reports 29946072 http://hdl.handle.net/20.500.12105/6575 SLEEP-APNEA DISEASE DYSBIOSIS IMMUNITY INFLAMMATION INFECTION BACTERIA LUNG CARCINOGENESIS INHIBITION Pulmonary Arterial Hypertension Affects the Rat Gut Microbiome