2026-06-14T06:44:10Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/65672024-10-31T11:47:36Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607
Repisalud
author
Toran, Jose Luis
author
Aguilar, Susana
author
Lopez, Juan Antonio
author
Torroja, Carlos
author
Quintana, Juan A.
author
Santiago, Cesar
author
Abad, José Luis
author
Gomes-Alves, Patricia
author
Gonzalez, Andres
author
Bernal, Juan Antonio
author
Jimenez-Borreguero, Luis J.
author
Alves, Paula Marques
author
R-Borlado, Luis
author
Vazquez, Jesus
author
Bernad, Antonio
funder
Unión Europea. Comisión Europea
funder
Ministerio de Ciencia e Innovación (España)
funder
Comunidad de Madrid (España)
funder
Instituto de Salud Carlos III
2018-11-05T08:30:14Z
2018-11-05T08:30:14Z
2017-10-02
Sci Rep. 2017; 7(1):12490
10.1038/s41598-017-11976-6
2045-2322
2045-2322
Scientific reports
28970523
http://hdl.handle.net/20.500.12105/6567
Studies in recent years have established that the principal effects in cardiac cell therapy are associated with paracrine/autocrine factors. We combined several complementary techniques to define human cardiac progenitor cell (CPC) secretome constituted by 914 proteins/genes; 51% of these are associated with the exosomal compartment. To define the set of proteins specifically or highly differentially secreted by CPC, we compared human mesenchymal stem cells and dermal fibroblasts; the study defined a group of growth factors, cytokines and chemokines expressed at high to medium levels by CPC. Among them, IL-1, GROa (CXCL1), CXCL6 (GCP2) and IL-8 are examples whose expression was confirmed by most techniques used. ELISA showed that CXCL6 is significantly overexpressed in CPC conditioned medium (CM) (18- to 26-fold) and western blot confirmed expression of its receptors CXCR1 and CXCR2. Addition of anti-CXCL6 completely abolished migration in CPC-CM compared with anti-CXCR2, which promoted partial inhibition, and anti-CXCR1, which was inefficient. Anti-CXCL6 also significantly inhibited CPC CM angiogenic activity. In vivo evaluation also supported a relevant role for angiogenesis. Altogether, these results suggest a notable angiogenic potential in CPC-CM and identify CXCL6 as an important paracrine factor for CPC that signals mainly through CXCR2.
eng
CXCL6
Cardiac progenitor cells
Proteomics
Secretome
CXCL6 is an important paracrine factor in the pro-angiogenic human cardiac progenitor-like cell secretome
journal article
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