2026-06-14T10:38:24Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/65672024-10-31T11:47:36Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607

00925njm 22002777a 4500 dc Toran, Jose Luis author Aguilar, Susana author Lopez, Juan Antonio author Torroja, Carlos author Quintana, Juan A. author Santiago, Cesar author Abad, José Luis author Gomes-Alves, Patricia author Gonzalez, Andres author Bernal, Juan Antonio author Jimenez-Borreguero, Luis J. author Alves, Paula Marques author R-Borlado, Luis author Vazquez, Jesus author Bernad, Antonio author 2017-10-02 Studies in recent years have established that the principal effects in cardiac cell therapy are associated with paracrine/autocrine factors. We combined several complementary techniques to define human cardiac progenitor cell (CPC) secretome constituted by 914 proteins/genes; 51% of these are associated with the exosomal compartment. To define the set of proteins specifically or highly differentially secreted by CPC, we compared human mesenchymal stem cells and dermal fibroblasts; the study defined a group of growth factors, cytokines and chemokines expressed at high to medium levels by CPC. Among them, IL-1, GROa (CXCL1), CXCL6 (GCP2) and IL-8 are examples whose expression was confirmed by most techniques used. ELISA showed that CXCL6 is significantly overexpressed in CPC conditioned medium (CM) (18- to 26-fold) and western blot confirmed expression of its receptors CXCR1 and CXCR2. Addition of anti-CXCL6 completely abolished migration in CPC-CM compared with anti-CXCR2, which promoted partial inhibition, and anti-CXCR1, which was inefficient. Anti-CXCL6 also significantly inhibited CPC CM angiogenic activity. In vivo evaluation also supported a relevant role for angiogenesis. Altogether, these results suggest a notable angiogenic potential in CPC-CM and identify CXCL6 as an important paracrine factor for CPC that signals mainly through CXCR2. Sci Rep. 2017; 7(1):12490 10.1038/s41598-017-11976-6 2045-2322 2045-2322 Scientific reports 28970523 http://hdl.handle.net/20.500.12105/6567 CXCL6 Cardiac progenitor cells Proteomics Secretome CXCL6 is an important paracrine factor in the pro-angiogenic human cardiac progenitor-like cell secretome