2026-05-20T22:56:54Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/65522024-11-29T18:30:20Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Misiewicz-Krzeminska, Irena
author
Corchete, Luis Antonio
author
Rojas, Elizabeta A
author
Martinez Lopez, Joaquin
author
García-Sanz, Ramón
author
Oriol, Albert
author
Bladé, Joan
author
Lahuerta, Juan José
author
Miguel, Jesús San
author
Mateos, María-Victoria
author
Gutiérrez, Norma C
funder
Junta de Castilla y León (España)
funder
Multiple Myeloma Research Foundation
2018-10-30T10:59:33Z
2018-10-30T10:59:33Z
2018-05
Haematologica. 2018; 103 (5) : 880 - 889.
10.3324/haematol.2017.181628
1592-8721
0390-6078
Haematologica
29545347
http://hdl.handle.net/20.500.12105/6552
Protein analysis in bone marrow samples from patients with multiple myeloma has been limited by the low concentration of proteins obtained after CD138+ cell selection. A novel approach based on capillary nano-immunoassay could make it possible to quantify dozens of proteins from each myeloma sample in an automated manner. Here we present a method for the accurate and robust quantification of the expression of multiple proteins extracted from CD138-purified multiple myeloma samples frozen in RLT Plus buffer, which is commonly used for nucleic acid preservation and isolation. Additionally, the biological and clinical value of this analysis for a panel of 12 proteins essential to the pathogenesis of multiple myeloma was evaluated in 63 patients with newly diagnosed multiple myeloma. The analysis of the prognostic impact of CRBN/Cereblon and IKZF1/Ikaros mRNA/protein showed that only the protein levels were able to predict progression-free survival of patients; mRNA levels were not associated with prognosis. Interestingly, high levels of Cereblon and Ikaros proteins were associated with longer progression-free survival only in patients who received immunomodulatory drugs and not in those treated with other drugs. In conclusion, the capillary nano-immunoassay platform provides a novel opportunity for automated quantification of the expression of more than 20 proteins in CD138+ primary multiple myeloma samples.
eng
DIAGNOSED MULTIPLE-MYELOMA
CEREBLON EXPRESSION
GENE-EXPRESSION
WESTERN BLOTS
LENALIDOMIDE
IDENTIFICATION
THALIDOMIDE
RNA
POMALIDOMIDE
ABUNDANCE
A novel nano-immunoassay method for quantification of proteins from CD138-purified myeloma cells: biological and clinical utility
journal article
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