2026-08-29T20:43:20Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/65522024-11-29T18:30:20Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597

00925njm 22002777a 4500 dc Misiewicz-Krzeminska, Irena author Corchete, Luis Antonio author Rojas, Elizabeta A author Martinez Lopez, Joaquin author García-Sanz, Ramón author Oriol, Albert author Bladé, Joan author Lahuerta, Juan José author Miguel, Jesús San author Mateos, María-Victoria author Gutiérrez, Norma C author 2018-05 Protein analysis in bone marrow samples from patients with multiple myeloma has been limited by the low concentration of proteins obtained after CD138+ cell selection. A novel approach based on capillary nano-immunoassay could make it possible to quantify dozens of proteins from each myeloma sample in an automated manner. Here we present a method for the accurate and robust quantification of the expression of multiple proteins extracted from CD138-purified multiple myeloma samples frozen in RLT Plus buffer, which is commonly used for nucleic acid preservation and isolation. Additionally, the biological and clinical value of this analysis for a panel of 12 proteins essential to the pathogenesis of multiple myeloma was evaluated in 63 patients with newly diagnosed multiple myeloma. The analysis of the prognostic impact of CRBN/Cereblon and IKZF1/Ikaros mRNA/protein showed that only the protein levels were able to predict progression-free survival of patients; mRNA levels were not associated with prognosis. Interestingly, high levels of Cereblon and Ikaros proteins were associated with longer progression-free survival only in patients who received immunomodulatory drugs and not in those treated with other drugs. In conclusion, the capillary nano-immunoassay platform provides a novel opportunity for automated quantification of the expression of more than 20 proteins in CD138+ primary multiple myeloma samples. Haematologica. 2018; 103 (5) : 880 - 889. 10.3324/haematol.2017.181628 1592-8721 0390-6078 Haematologica 29545347 http://hdl.handle.net/20.500.12105/6552 DIAGNOSED MULTIPLE-MYELOMA CEREBLON EXPRESSION GENE-EXPRESSION WESTERN BLOTS LENALIDOMIDE IDENTIFICATION THALIDOMIDE RNA POMALIDOMIDE ABUNDANCE A novel nano-immunoassay method for quantification of proteins from CD138-purified myeloma cells: biological and clinical utility