2026-05-17T05:43:41Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/65292024-09-27T08:58:07Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Thottacherry, Joseph Jose
author
Kosmalska, Anita Joanna
author
Kumar, Amit
author
Vishen, Amit Singh
author
Elosegui-Artola, Alberto
author
Pradhan, Susav
author
Sharma, Sumit
author
Singh, Parvinder P.
author
Guadamillas, Marta C.
author
Chaudhary, Natasha
author
Vishwakarma, Ram
author
Trepat, Xavier
author
del Pozo, Miguel Angel
author
Parton, Robert G.
author
Rao, Madan
author
Pullarkat, Pramod
author
Roca-Cusachs, Pere
author
Mayor, Satyajit
funder
Ministerio de Economía y Competitividad (España)
funder
Government of Catalonia (España)
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
Unión Europea. Comisión Europea
funder
Fundación La Caixa
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Fundación La Marató TV3
funder
National Health and Medical Research Council (Australia)
funder
Australian Research Council
funder
Council for Scientific and Industrial Research (India)
funder
Wellcome Trust
2018-10-26T07:59:24Z
2018-10-26T07:59:24Z
2018
Nat Commun. 2018; 9(1):4217
10.1038/s41467-018-06738-5
2041-1723
Nature Communications
30310066
http://hdl.handle.net/20.500.12105/6529
Plasma membrane tension regulates many key cellular processes. It is modulated by, and can modulate, membrane trafficking. However, the cellular pathway(s) involved in this interplay is poorly understood. Here we find that, among a number of endocytic processes operating simultaneously at the cell surface, a dynamin independent pathway, the CLIC/GEEC (CG) pathway, is rapidly and specifically upregulated upon a sudden reduction of tension. Moreover, inhibition (activation) of the CG pathway results in lower (higher) membrane tension. However, alteration in membrane tension does not directly modulate CG endocytosis. This requires vinculin, a mechano-transducer recruited to focal adhesion in adherent cells. Vinculin acts by controlling the levels of a key regulator of the CG pathway, GBF1, at the plasma membrane. Thus, the CG pathway directly regulates membrane tension and is in turn controlled via a mechano-chemical feedback inhibition, potentially leading to homeostatic regulation of membrane tension in adherent cells.
eng
CLATHRIN-MEDIATED ENDOCYTOSIS
FOCAL ADHESION DYNAMICS
TETHER FORMATION
MECHANOTRANSDUCTION
VINCULIN
ACTIVATION
FORCE
ENDOSOMES
INTEGRIN
PATHWAY
Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells
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