2026-05-17T05:43:40Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/65292024-09-27T08:58:07Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Thottacherry, Joseph Jose author Kosmalska, Anita Joanna author Kumar, Amit author Vishen, Amit Singh author Elosegui-Artola, Alberto author Pradhan, Susav author Sharma, Sumit author Singh, Parvinder P. author Guadamillas, Marta C. author Chaudhary, Natasha author Vishwakarma, Ram author Trepat, Xavier author del Pozo, Miguel Angel author Parton, Robert G. author Rao, Madan author Pullarkat, Pramod author Roca-Cusachs, Pere author Mayor, Satyajit author 2018 Plasma membrane tension regulates many key cellular processes. It is modulated by, and can modulate, membrane trafficking. However, the cellular pathway(s) involved in this interplay is poorly understood. Here we find that, among a number of endocytic processes operating simultaneously at the cell surface, a dynamin independent pathway, the CLIC/GEEC (CG) pathway, is rapidly and specifically upregulated upon a sudden reduction of tension. Moreover, inhibition (activation) of the CG pathway results in lower (higher) membrane tension. However, alteration in membrane tension does not directly modulate CG endocytosis. This requires vinculin, a mechano-transducer recruited to focal adhesion in adherent cells. Vinculin acts by controlling the levels of a key regulator of the CG pathway, GBF1, at the plasma membrane. Thus, the CG pathway directly regulates membrane tension and is in turn controlled via a mechano-chemical feedback inhibition, potentially leading to homeostatic regulation of membrane tension in adherent cells. Nat Commun. 2018; 9(1):4217 10.1038/s41467-018-06738-5 2041-1723 Nature Communications 30310066 http://hdl.handle.net/20.500.12105/6529 CLATHRIN-MEDIATED ENDOCYTOSIS FOCAL ADHESION DYNAMICS TETHER FORMATION MECHANOTRANSDUCTION VINCULIN ACTIVATION FORCE ENDOSOMES INTEGRIN PATHWAY Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells