2026-08-12T20:33:22Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/65212024-09-27T09:15:21Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Bhardwaj, Deepshikha
author
Nager, Mireia
author
Camats, Judith
author
David, Monica
author
Benguria, Alberto
author
Dopazo, Ana
author
Canti, Carles
author
Herreros, Judit
funder
Instituto de Salud Carlos III
funder
Government of Catalonia (España)
2018-10-25T08:19:46Z
2018-10-25T08:19:46Z
2013
Front Cell Neurosci. 2013; 7:52
10.3389/fncel.2013.00052
1662-5102
Frontiers in Cellular Neuroscience
23641195
http://hdl.handle.net/20.500.12105/6521
Axon morphogenesis is a complex process regulated by a variety of secreted molecules, including morphogens and growth factors, resulting in the establishment of the neuronal circuitry. Our previous work demonstrated that growth factors [Neurotrophins (NT) and Hepatocyte Growth Factor (HGF)] signal through beta-catenin during axon morphogenesis. HGF signaling promotes axon outgrowth and branching by inducing beta-catenin phosphorylation at Y142 and transcriptional regulation of T-Cell Factor (TCF) target genes. Here, we asked which genes are regulated by HGF signaling during axon morphogenesis. An array screening indicated that HGF signaling elevates the expression of chemokines of the CC and CXC families. In line with this, CCL7, CCL20, and CXCL2 significantly increase axon outgrowth in hippocampal neurons. Experiments using blocking antibodies and chemokine receptor antagonists demonstrate that chemokines act downstream of HGF signaling during axon morphogenesis. In addition, qPCR data demonstrates that CXCL2 and CCL5 expression is stimulated by HGF through Met/b-catenin/TCF pathway. These results identify CC family members and CXCL2 chemokines as novel regulators of axon morphogenesis downstream of HGF signaling.
eng
Beta-catenin
Axon
Neurite outgrowth
Chemokine
Hippocampal neurons
Hepatocyte growth factor
CELL-DERIVED FACTOR-1
BETA-CATENIN
INTERNEURON MIGRATION
HIPPOCAMPAL-NEURONS
NEURITE OUTGROWTH
SYNAPSE FORMATION
NERVOUS-SYSTEM
DENTATE GYRUS
RECEPTORS
CXCR4
Chemokines induce axon outgrowth downstream of Hepatocyte Growth Factor and TCF/beta-catenin signaling
journal article
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ChemokinesInduceAxonOutgrowth_2013.pdf
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83016e4626f9d2774f9c8975f87e5458
49080
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ChemokinesInduceAxonOutgrowth_2013.pdf.txt