2026-04-28T21:35:04Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/65172024-09-27T08:07:25Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Redondo, Santiago
author
Navarro-Dorado, Jorge
author
Ramajo, Marta
author
Medina, Ursula
author
Molina-Sanchez, Pedro
author
Garces, Zaady
author
Garcia-Alonso, Mauricio
author
Reguillo, Fernando
author
Rodriguez, Enrique
author
Andres, Vicente
author
Tejerina, Teresa
2018-10-25T08:19:45Z
2018-10-25T08:19:45Z
2014
J Cardiothorac Surg. 2014; 9(1):24
1749-8090
http://hdl.handle.net/20.500.12105/6517
24495866
10.1186/1749-8090-9-24
Journal of Cardiothoracic Surgery
Background: Transforming growth factor beta (TGF-beta 1) is a pleiotropic cytokine, which is deregulated in atherosclerosis; however the role of age in this process is unknown. We aimed to assess whether TGF-beta 1 signaling is affected by age. Methods: Vascular smooth muscle cells (VSMC) were obtained from patients undergoing abdominal surgery. Levels of TGF-beta 1 were measured by ELISA in sera from 169 patients undergoing coronary artery bypass grafting (CABG). The p27 expression was determined by Western blot from internal mammary arteries (IMA) obtained from CABG patients (n = 13). In VSMC from these patients undergoing abdominal surgery, secretion of TGF-beta 1 was determined by ELISA of cell-conditioned media. Results: In VSMC from aged patients we observed a lower TGF-beta 1 secretion, measured as TGF-beta 1 concentration in cell conditioned medium (p < 0.001). This effect was correlated to an age-dependent decrease of p27 expression in IMA from aged CABG patients. In a similar manner, there was an age-dependent decrease of serum TGF-beta 1 levels in CABG patients (p = 0.0195). Conclusions: VSMC from aged patients showed a higher degree of cellular senescence and it was associated to a lower TGF-beta 1 secretion and signaling.
eng
Growth factors
Coronary disease
Aging
Surgery
GROWTH-FACTOR-BETA
TGF-BETA
ATHEROSCLEROSIS
P27(KIP1)
DISEASE
PROLIFERATION
INHIBITOR
PLASMA
BLOOD
CELLS
Age-dependent defective TGF-beta1 signaling in patients undergoing coronary artery bypass grafting
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