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oai:repisalud.isciii.es:20.500.12105/65172024-09-27T08:07:25Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Redondo, Santiago author Navarro-Dorado, Jorge author Ramajo, Marta author Medina, Ursula author Molina-Sanchez, Pedro author Garces, Zaady author Garcia-Alonso, Mauricio author Reguillo, Fernando author Rodriguez, Enrique author Andres, Vicente author Tejerina, Teresa author 2014 Background: Transforming growth factor beta (TGF-beta 1) is a pleiotropic cytokine, which is deregulated in atherosclerosis; however the role of age in this process is unknown. We aimed to assess whether TGF-beta 1 signaling is affected by age. Methods: Vascular smooth muscle cells (VSMC) were obtained from patients undergoing abdominal surgery. Levels of TGF-beta 1 were measured by ELISA in sera from 169 patients undergoing coronary artery bypass grafting (CABG). The p27 expression was determined by Western blot from internal mammary arteries (IMA) obtained from CABG patients (n = 13). In VSMC from these patients undergoing abdominal surgery, secretion of TGF-beta 1 was determined by ELISA of cell-conditioned media. Results: In VSMC from aged patients we observed a lower TGF-beta 1 secretion, measured as TGF-beta 1 concentration in cell conditioned medium (p < 0.001). This effect was correlated to an age-dependent decrease of p27 expression in IMA from aged CABG patients. In a similar manner, there was an age-dependent decrease of serum TGF-beta 1 levels in CABG patients (p = 0.0195). Conclusions: VSMC from aged patients showed a higher degree of cellular senescence and it was associated to a lower TGF-beta 1 secretion and signaling. J Cardiothorac Surg. 2014; 9(1):24 1749-8090 http://hdl.handle.net/20.500.12105/6517 24495866 10.1186/1749-8090-9-24 Journal of Cardiothoracic Surgery Growth factors Coronary disease Aging Surgery GROWTH-FACTOR-BETA TGF-BETA ATHEROSCLEROSIS P27(KIP1) DISEASE PROLIFERATION INHIBITOR PLASMA BLOOD CELLS Age-dependent defective TGF-beta1 signaling in patients undergoing coronary artery bypass grafting