2026-06-14T03:29:40Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/64982024-10-31T12:00:21Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Jose Blanco, Maria
author
Rodriguez-Martin, Ivan
author
Learte, Ana I. R.
author
Clemente, Cristina
author
Montalvo, Maria Gregoria
author
Seiki, Motoharu
author
Arroyo, Alicia G
author
Sanchez-Camacho, Cristina
funder
European University of Madrid (España)
funder
Ministerio de Economía, Industria y Competitividad (España)
funder
Fundación ProCNIC
2018-10-19T08:00:43Z
2018-10-19T08:00:43Z
2017
PLoS One. 2017; 12(9):e0184767
10.1371/journal.pone.0184767
1932-6203
PLoS ONE
28926609
http://hdl.handle.net/20.500.12105/6498
Matrix metalloproteinases (MMPs) constitute a large group of
endoproteases that play important functions during embryonic
development, tumor metastasis and angiogenesis by degrading components
of the extracellular matrix. Within this family, we focused our study on
Mt4-mmp (also called Mmp17) that belongs to a distinct subset that is
anchored to the cell surface via a glycosylphosphatidylinositol (GPI)
moiety and with the catalytic site exposed to the extracellular space.
Information about its function and substrates is very limited to date,
and little has been reported on its role in the developing embryo. Here,
we report a detailed expression analysis of Mt4-mmp during mouse
embryonic development by using a LacZ reporter transgenic mouse line. We
showed that Mt4-mmp is detected from early stages of development to
postnatal stages following a dynamic and restricted pattern of
expression. Mt4-mmp was first detected at E8.5 limited to the
intersomitic vascularization, the endocardial endothelium and the dorsal
aorta. Mt4-mmp(LacZ/+) cells were also observed in the neural crest
cells, somites, floor plate and notochord at early stages. From E10.5,
expression localized in the limb buds and persists during limb
development. A strong expression in the brain begins at E12.5 and
continues to postnatal stages. Specifically, staining was observed in
the olfactory bulb, cerebral cortex, hippocampus, striatum, septum,
dorsal thalamus and the spinal cord. In addition, LacZ-positive cells
were also detected during eye development, initially at the hyaloid
artery and later on located in the lens and the neural retina. Mt4-mmp
expression was confirmed by quantitative RT-PCR and western blot
analysis in some embryonic tissues. Our data point to distinct functions
for this metalloproteinase during embryonic development, particularly
during brain formation, angiogenesis and limb development.
eng
SOMITE-DERIVED ANGIOBLASTS
OSTEOPONTIN MESSENGER-RNA
DEVELOPING
RAT-BRAIN
MATRIX METALLOPROTEINASES
PROTEOLYTIC ACTIVITY
SONIC-HEDGEHOG
AXON GUIDANCE
IN-VIVO
LIMB
CANCER
Developmental expression of membrane type 4-matrix metalloproteinase
(Mt4-mmp/Mmp17) in the mouse embryo
journal article
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