2026-06-14T03:32:00Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/64882025-05-08T13:13:48Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2052col_20.500.12105_16938col_20.500.12105_19616col_20.500.12105_19619

00925njm 22002777a 4500 dc Matamala, Nerea author Aggarwal, Nupur author Iadarola, Paolo author Fumagalli, Marco author Gomez-Mariano, Gema Maria author Lara, Beatriz author Varela Martinez, Maria del Carmen author Cuesta de la Plaza, Isabel author Stolk, Jan author Janciauskiene, Sabina author Martinez-Delgado, Beatriz author 2017 Human SERPINA1 gene is located on chromosome 14q31-32.3 and is organized into three (IA, IB, and IC) non-coding and four (II, III, IV, V) coding exons. This gene produces α1-antitrypsin (A1AT), a prototypical member of the serpin superfamily of proteins. We demonstrate that human peripheral blood leukocytes express not only a product corresponding to the transcript coding for the full-length A1AT protein but also two short transcripts (ST1C4 and ST1C5) of A1AT. In silico sequence analysis revealed that the last exon of the short transcripts contains an Open Reading Frame (ORF) and thus putatively can produce peptides. We found ST1C4 expression across different human tissues whereas ST1C5 was mainly restricted to leukocytes, specifically neutrophils. A high up-regulation (10-fold) of short transcripts was observed in isolated human blood neutrophils after activation with lipopolysaccharide. Parallel analyses by liquid chromatography-mass spectrometry identified peptides corresponding to C-terminal region of A1AT in supernatants of activated but not naïve neutrophils. Herein we report for the first time a tissue specific expression and regulation of short transcripts of SERPINA1 gene, and the presence of C-terminal peptides in supernatants from activated neutrophils, in vitro. This gives a novel insight into the studies on the transcription of SERPINA1 gene. PLoS One. 2017 Jan 20;12(1):e0170533. 10.1371/journal.pone.0170533 1932-6203 1932-6203 PloS one 28107454 http://hdl.handle.net/20.500.12105/6488 Identification of Novel Short C-Terminal Transcripts of Human SERPINA1 Gene