2026-06-14T03:36:02Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/56532024-05-27T14:19:49Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597

00925njm 22002777a 4500 dc Zhou, Jing author Benito-Martin, Alberto author Mighty, Jason author Chang, Lynne author Ghoroghi, Shima author Wu, Hao author Wong, Madeline author Guariglia, Sara author Baranov, Petr author Young, Michael author Gharbaran, Rajendra author Emerson, Mark author Mark, Milica Tesic author Molina, Henrik author Canto-Solar, M Valeria author Peinado Selgas , Hector author Redenti, Stephen author 2018-02-12 A range of cell types, including embryonic stem cells, neurons and astrocytes have been shown to release extracellular vesicles (EVs) containing molecular cargo. Across cell types, EVs facilitate transfer of mRNA, microRNA and proteins between cells. Here we describe the release kinetics and content of EVs from mouse retinal progenitor cells (mRPCs). Interestingly, mRPC derived EVs contain mRNA, miRNA and proteins associated with multipotency and retinal development. Transcripts enclosed in mRPC EVs, include the transcription factors Pax6, Hes1, and Sox2, a mitotic chromosome stabilizer Ki67, and the neural intermediate filaments Nestin and GFAP. Proteomic analysis of EV content revealed retinogenic growth factors and morphogen proteins. mRPC EVs were shown to transfer GFP mRNA between cell populations. Finally, analysis of EV mediated functional cargo delivery, using the Cre-loxP recombination system, revealed transfer and uptake of Cre+ EVs, which were then internalized by target mRPCs activating responder loxP GFP expression. In summary, the data supports a paradigm of EV genetic material encapsulation and transfer within RPC populations. RPC EV transfer may influence recipient RPC transcriptional and post-transcriptional regulation, representing a novel mechanism of differentiation and fate determination during retinal development. Sci Rep. 2018;8(1):2823. 10.1038/s41598-018-20421-1 2045-2322 2045-2322 Scientific reports 29434302 http://hdl.handle.net/20.500.12105/5653 ROD PHOTORECEPTOR DIFFERENTIATION STEM-CELLS IN-VITRO GENE-EXPRESSION MESSENGER-RNA FATE DETERMINATION MEDIATED TRANSFER APOPTOTIC BODIES MOUSE RETINA BODY-FLUIDS Retinal progenitor cells release extracellular vesicles containing developmental transcription factors, microRNA and membrane proteins