2026-04-29T15:25:27Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/55392024-09-27T09:29:19Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Rodius, Sophie
author
Nazarov, Petr V.
author
Nepomuceno-Chamorro, Isabel A.
author
Jeanty, Celine
author
Gonzalez-Rosa, Juan Manuel
author
Ibberson, Mark
author
da Costa, Ricardo M. Benites
author
Xenarios, Ioannis
author
Mercader, Nadia
author
Azuaje, Francisco
funder
Luxembourg National Research Fund
funder
Swiss National Research Foundation
funder
INFUSED project
funder
Ministerio de Economía y Competitividad (España)
funder
Comunidad de Madrid (España)
2017-12-01T07:37:29Z
2017-12-01T07:37:29Z
2014
BMC Genomics. 2014; 15(1):852
10.1186/1471-2164-15-852
1471-2164
BMC Genomics
http://hdl.handle.net/20.500.12105/5539
Background: Zebrafish is a clinically-relevant model of heart regeneration. Unlike mammals, it has a remarkable heart repair capacity after injury, and promises novel translational applications. Amputation and cryoinjury models are key research tools for understanding injury response and regeneration in vivo. An understanding of the transcriptional responses following injury is needed to identify key players of heart tissue repair, as well as potential targets for boosting this property in humans. Results: We investigated amputation and cryoinjury in vivo models of heart damage in the zebrafish through unbiased, integrative analyses of independent molecular datasets. To detect genes with potential biological roles, we derived computational prediction models with microarray data from heart amputation experiments. We focused on a top-ranked set of genes highly activated in the early post-injury stage, whose activity was further verified in independent microarray datasets. Next, we performed independent validations of expression responses with qPCR in a cryoinjury model. Across in vivo models, the top candidates showed highly concordant responses at 1 and 3 days post-injury, which highlights the predictive power of our analysis strategies and the possible biological relevance of these genes. Top candidates are significantly involved in cell fate specification and differentiation, and include heart failure markers such as periostin, as well as potential new targets for heart regeneration. For example, ptgis and ca2 were overexpressed, while usp2a, a regulator of the p53 pathway, was down-regulated in our in vivo models. Interestingly, a high activity of ptgis and ca2 has been previously observed in failing hearts from rats and humans. Conclusions: We identified genes with potential critical roles in the response to cardiac damage in the zebrafish. Their transcriptional activities are reproducible in different in vivo models of cardiac injury.
eng
Myocardial infarction
Zebrafish
Ventricular amputation
Ventricular cryoinjury
Heart regeneration
Transcriptional responses
Transcriptional association networks
INDUCED MYOCARDIAL-INFARCTION
HEART REGENERATION
NEMALINE MYOPATHY
HYPERTROPHIC CARDIOMYOPATHY
STEM-CELLS
CARDIOVASCULAR-SYSTEM
INTERACTION NETWORKS
EXPRESSION
PROLIFERATION
PERIOSTIN
Transcriptional response to cardiac injury in the zebrafish: systematic identification of genes with highly concordant activity across in vivo models
journal article
URL
https://repisalud.isciii.es/bitstreams/e3e79f1d-3fdc-4fc8-88a2-53ab9968a355/download
File
MD5
7f08629ddac34c801b09dd192132e1cb
1994115
application/pdf
TranscriptionalResponseToCardiac_2014.pdf
URL
https://repisalud.isciii.es/bitstreams/20c3d025-496a-418f-9f4d-a2e7851aa9d3/download
File
MD5
f2630e19c5e3629f699fac0df9824c7a
69265
text/plain
TranscriptionalResponseToCardiac_2014.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/26fc8d2b-fdae-477d-a2b3-d60eb8526903/download
File
MD5
f2630e19c5e3629f699fac0df9824c7a
69265
text/plain
TranscriptionalResponseToCardiac_2014.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/5058dcb8-5a6b-4f3d-810d-50569163d782/download
File
MD5
c2d67eacebf7136b623c7a07343dd46d
69252
text/plain
TranscriptionalResponseToCardiac_2014.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/13ae0928-2dc0-4848-8ede-46fda6ae2e06/download
File
MD5
c2d67eacebf7136b623c7a07343dd46d
69252
text/plain
TranscriptionalResponseToCardiac_2014.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/c4280b24-7996-401d-96d6-fdbb4f5745ed/download
File
MD5
f2630e19c5e3629f699fac0df9824c7a
69265
text/plain
TranscriptionalResponseToCardiac_2014.pdf.txt