2026-06-14T03:36:20Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/55322024-10-31T11:39:22Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Li, Hongzhe
author
Ghazanfari, Roshanak
author
Zacharaki, Dimitra
author
Ditzel, Nicholas
author
Isern, Joan
author
Ekblom, Marja
author
Mendez-Ferrer, Simon
author
Kassem, Moustapha
author
Scheding, Stefan
funder
Swedish Research Council
funder
Swedish Cancer Foundation
funder
Swedish Childhood Cancer Foundation
funder
Gunnar Nilsson's Cancer Foundation
funder
Gunnel Bjork's Testament
funder
Skane County Council's Research Foundation
2017-12-01T07:37:28Z
2017-12-01T07:37:28Z
2014
Stem Cell Reports. 2014; 3(6):965-74
10.1016/j.stemcr.2014.09.018
2213-6711
Stem Cell Reports
25454633
http://hdl.handle.net/20.500.12105/5532
Human bone marrow (BM) contains a rare population of nonhematopoietic mesenchymal stromal cells (MSCs), which are of central importance for the hematopoietic microenvironment. However, the precise phenotypic definition of these cells in adult BM has not yet been reported. In this study, we show that low/negative expression of CD140a (PDGFR-alpha) on lin(-)/CD45(-)/CD271(+) BM cells identified a cell population with very high MSC activity, measured as fibroblastic colony-forming unit frequency and typical in vitro and in vivo stroma formation and differentiation capacities. Furthermore, these cells exhibited high levels of genes associated with mesenchymal lineages and HSC supportive function. Moreover, lin(-)/CD45(-)/CD271(+)/CD140a(low/-) cells effectively mediated the ex vivo expansion of transplantable CD34(+) hematopoietic stem cells. Taken together, these data indicate that CD140a is a key negative selection marker for adult human BM-MSCs, which enables to prospectively isolate a close to pure population of candidate human adult stroma stem/progenitor cells with potent hematopoiesis-supporting capacity.
eng
STEM-CELLS
EXPANSION
Low/Negative Expression of PDGFR-alpha Identifies the Candidate Primary Mesenchymal Stromal Cells in Adult Human Bone Marrow
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