2026-06-14T03:30:18Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/55302024-09-27T08:51:46Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Gonzalez-Rosa, Juan Manuel author Guzman-Martinez, Gabriela author Marques, Ines Joao author Sanchez-Iranzo, Hector author Jimenez-Borreguero, Luis J. author Mercader, Nadia author 2014 Aims: While zebrafish embryos are amenable to in vivo imaging, allowing the study of morphogenetic processes during development, intravital imaging of adults is hampered by their small size and loss of transparency. The use of adult zebrafish as a vertebrate model of cardiac disease and regeneration is increasing at high speed. It is therefore of great importance to establish appropriate and robust methods to measure cardiac function parameters. Methods and Results: Here we describe the use of 2D-echocardiography to study the fractional volume shortening and segmental wall motion of the ventricle. Our data show that 2D-echocardiography can be used to evaluate cardiac injury and also to study recovery of cardiac function. Interestingly, our results show that while global systolic function recovered following cardiac cryoinjury, ventricular wall motion was only partially restored. Conclusion: Cryoinjury leads to long-lasting impairment of cardiac contraction, partially mimicking the consequences of myocardial infarction in humans. Functional assessment of heart regeneration by echocardiography allows a deeper understanding of the mechanisms of cardiac regeneration and has the advantage of being easily transferable to other cardiovascular zebrafish disease models. PLoS One. 2014; 9(12):e115604 10.1371/journal.pone.0115604 1932-6203 PLoS One 25532015 http://hdl.handle.net/20.500.12105/5530 HEART REGENERATION ADULT ZEBRAFISH MYOCARDIAL-INFARCTION CARDIAC REGENERATION GENE REPRODUCIBILITY EXPRESSION DISEASE Use of Echocardiography Reveals Reestablishment of Ventricular Pumping Efficiency and Partial Ventricular Wall Motion Recovery upon Ventricular Cryoinjury in the Zebrafish