2026-06-14T03:30:15Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/55262024-09-27T08:04:31Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Martinez-Lopez, Maria
author
Iborra, Salvador
author
Conde-Garrosa, Ruth
author
Sancho, David
funder
Ministerio de Economía y Competitividad (España)
funder
Unión Europea. Comisión Europea
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
Ministerio de Educación (España)
2017-12-01T07:37:27Z
2017-12-01T07:37:27Z
2015
Eur J Immunol. 2015; 45(1):119-29
10.1002/eji.201444651
1521-4141
0014-2980
European Journal of Immunology
25312824
http://hdl.handle.net/20.500.12105/5526
The role of different DC subsets in priming and maintenance of immunity against Leishmania major (L. major) infection is debated. The transcription factor basic leucine zipper transcription factor, ATF-like 3 (Batf3) is essential for the development of mouse CD103(+) DCs and some functions of CD8(+) DCs. We found that CD103(+) DCs were significantly reduced in the dermis of Batf3-deficient C57BL/6 mice. Batf3(-/-) mice developed exacerbated and unresolved cutaneous pathology following a low dose of intradermal L. major infection in the ear pinnae. Parasite load was increased 1000-fold locally and expanded systemically. Batf3 deficiency did not affect L. major antigen presentation to T cells, which was directly exerted by CD8(-) conventional DCs (cDCs) in the skin draining LN. However, CD4(+) T-cell differentiation in the LN and skin was skewed to nonprotective Treg- and Th2-cell subtypes. CD103(+) DCs are major IL-12 producers during L. major infection. Local Th1 immunity was severely hindered, correlating with impaired IL-12 production and reduction in CD103(+) DC numbers. Adoptive transfer of WT but not IL-12p40(-/-) Batf3-dependent DCs significantly improved anti-L. major response in infected Batf3(-/-) mice. Our results suggest that IL-12 production by Batf3-dependent CD103(+) DCs is crucial for maintenance of local Th1 immunity against L. major infection.
eng
Adaptive immune response
Batf3
Dendritic cells
IL-12
Leishmania major
T-CELLS
CROSS-PRESENTATION
LANGERHANS CELLS
CUTTING EDGE
IN-VIVO
RESPONSES
SKIN
CD8(+)
INTERLEUKIN-12
MACROPHAGES
Batf3-dependent CD103(+) dendritic cells are major producers of IL-12 that drive local Th1 immunity against Leishmania major infection in mice
journal article
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Batf3-dependentCD103Dendritic_2015.pdf
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Batf3-dependentCD103Dendritic_2015_supplFig.pdf
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Batf3-dependentCD103Dendritic_2015.pdf.txt
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Batf3-dependentCD103Dendritic_2015_supplFig.pdf.txt