2026-06-14T03:31:55Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/55262024-09-27T08:04:31Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Martinez-Lopez, Maria author Iborra, Salvador author Conde-Garrosa, Ruth author Sancho, David author 2015 The role of different DC subsets in priming and maintenance of immunity against Leishmania major (L. major) infection is debated. The transcription factor basic leucine zipper transcription factor, ATF-like 3 (Batf3) is essential for the development of mouse CD103(+) DCs and some functions of CD8(+) DCs. We found that CD103(+) DCs were significantly reduced in the dermis of Batf3-deficient C57BL/6 mice. Batf3(-/-) mice developed exacerbated and unresolved cutaneous pathology following a low dose of intradermal L. major infection in the ear pinnae. Parasite load was increased 1000-fold locally and expanded systemically. Batf3 deficiency did not affect L. major antigen presentation to T cells, which was directly exerted by CD8(-) conventional DCs (cDCs) in the skin draining LN. However, CD4(+) T-cell differentiation in the LN and skin was skewed to nonprotective Treg- and Th2-cell subtypes. CD103(+) DCs are major IL-12 producers during L. major infection. Local Th1 immunity was severely hindered, correlating with impaired IL-12 production and reduction in CD103(+) DC numbers. Adoptive transfer of WT but not IL-12p40(-/-) Batf3-dependent DCs significantly improved anti-L. major response in infected Batf3(-/-) mice. Our results suggest that IL-12 production by Batf3-dependent CD103(+) DCs is crucial for maintenance of local Th1 immunity against L. major infection. Eur J Immunol. 2015; 45(1):119-29 10.1002/eji.201444651 1521-4141 0014-2980 European Journal of Immunology 25312824 http://hdl.handle.net/20.500.12105/5526 Adaptive immune response Batf3 Dendritic cells IL-12 Leishmania major T-CELLS CROSS-PRESENTATION LANGERHANS CELLS CUTTING EDGE IN-VIVO RESPONSES SKIN CD8(+) INTERLEUKIN-12 MACROPHAGES Batf3-dependent CD103(+) dendritic cells are major producers of IL-12 that drive local Th1 immunity against Leishmania major infection in mice