2026-06-14T05:08:13Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/54122024-10-31T11:58:28Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607
Repisalud
author
Ayllon, Nieves
author
Villar, Margarita
author
Galindo, Ruth C.
author
Kocan, Katherine M.
author
Sima, Radek
author
Lopez, Juan Antonio
author
Vazquez, Jesus
author
Alberdi, Pilar
author
Cabezas-Cruz, Alejandro
author
Kopacek, Petr
author
de la Fuente, Jose
funder
Unión Europea. Comisión Europea
funder
Oklahoma Agricultural Experiment
funder
Ministerio de Economía y Competitividad (España)
2017-11-27T13:49:53Z
2017-11-27T13:49:53Z
2015
PLoS Genet. 2015; 11(3):e1005120
10.1371/journal.pgen.1005120
1553-7404
PLOS GENETICS
25815810
http://hdl.handle.net/20.500.12105/5412
Anaplasma phagocytophilum is an emerging pathogen that causes human
granulocytic anaplasmosis. Infection with this zoonotic pathogen affects
cell function in both vertebrate host and the tick vector, Ixodes
scapularis. Global tissue-specific response and apoptosis signaling
pathways were characterized in I. scapularis nymphs and adult female
midguts and salivary glands infected with A. phagocytophilum using a
systems biology approach combining transcriptomics and proteomics.
Apoptosis was selected for pathway-focused analysis due to its role in
bacterial infection of tick cells. The results showed tissue-specific
differences in tick response to infection and revealed differentiated
regulation of apoptosis pathways. The impact of bacterial infection was
more pronounced in tick nymphs and midguts than in salivary glands,
probably reflecting bacterial developmental cycle. All apoptosis
pathways described in other organisms were identified in I. scapularis,
except for the absence of the Perforin ortholog. Functional
characterization using RNA interference showed that Porin knockdown
significantly increases tick colonization by A. phagocytophilum.
Infection with A. phagocytophilum produced complex tissue-specific
alterations in transcript and protein levels. In tick nymphs, the
results suggested a possible effect of bacterial infection on the
inhibition of tick immune response. In tick midguts, the results
suggested that A. phagocytophilum infection inhibited cell apoptosis to
facilitate and establish infection through up-regulation of the JAK/STAT
pathway. Bacterial infection inhibited the intrinsic apoptosis pathway
in tick salivary glands by down-regulating Porin expression that
resulted in the inhibition of Cytochrome c release as the anti-apoptotic
mechanism to facilitate bacterial infection. However, tick salivary
glands may promote apoptosis to limit bacterial infection through
induction of the extrinsic apoptosis pathway. These dynamic changes in
response to A. phagocytophilum in I. scapularis tissue-specific
transcriptome and proteome demonstrated the complexity of the tick
response to infection and will contribute to characterize gene
regulation in ticks.
eng
FATTY-ACID SYNTHASE
GENE-EXPRESSION
QUANTITATIVE PROTEOMICS
HUMAN
NEUTROPHILS
CANCER-CELLS
RNA-SEQ
INFECTION
INHIBITORS
LIKELIHOOD
EHRLICHIA
Systems Biology of Tissue-Specific Response to Anaplasma phagocytophilum
Reveals Differentiated Apoptosis in the Tick Vector Ixodes scapularis
journal article
URL
https://repisalud.isciii.es/bitstreams/0145c18c-ba55-4b2e-a658-ab3dce8d21eb/download
File
MD5
f28e9bfd046c3f714418da99a4d14f60
7392491
application/pdf
SystemsBiologyOfTissue-Specific_2015.pdf
URL
https://repisalud.isciii.es/bitstreams/d68724f9-84d7-4e65-9bda-4528aed961f7/download
File
MD5
7f15d2598c0ff7629ebf43141f984b85
3007216
image/tiff
SystemsBiologyOfTissue-Specific_2015_S1_Fig.tif
URL
https://repisalud.isciii.es/bitstreams/5ad795a3-4bef-4e8c-8683-e155085441d3/download
File
MD5
3b5f923466a8487167fd6c9ee171f676
44092
application/pdf
SystemsBiologyOfTissue-Specific_2015_S1_Table.pdf
URL
https://repisalud.isciii.es/bitstreams/03bec68a-7bea-4a05-884f-a60c46689f27/download
File
MD5
882fdbc1ca16cc08e2ac1490abd3e44c
3006888
image/tiff
SystemsBiologyOfTissue-Specific_2015_S2_Fig.tif
URL
https://repisalud.isciii.es/bitstreams/47a6d6bc-f138-45b9-af95-d44a2d7f3096/download
File
MD5
1aaee0aa145edb65677a70e7a7f0439e
3011398
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
SystemsBiologyOfTissue-Specific_2015_S2_Table.xlsx
URL
https://repisalud.isciii.es/bitstreams/29342a07-910a-4ef2-acd4-f5a85049a512/download
File
MD5
1aaee0aa145edb65677a70e7a7f0439e
3011398
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
SystemsBiologyOfTissue-Specific_2015_S2_Table.xlsx
URL
https://repisalud.isciii.es/bitstreams/0c5fb6c1-f14e-4c2a-9524-b9b67e31e9e2/download
File
MD5
7d43bcded8676a6b71378a7b959c4a43
3006868
image/tiff
SystemsBiologyOfTissue-Specific_2015_S3_Fig.tif
URL
https://repisalud.isciii.es/bitstreams/b1343760-5306-44bb-af16-80ea2739f1e9/download
File
MD5
5619569cfd7b920382f27fdc841b9e3d
896279
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
SystemsBiologyOfTissue-Specific_2015_S3_Table.xlsx
URL
https://repisalud.isciii.es/bitstreams/faacc325-57db-42dc-a141-5c0d650146f4/download
File
MD5
2b558ef9b6553c3cddcb926239f18c61
3007313
image/tiff
SystemsBiologyOfTissue-Specific_2015_S4_Fig.tif
URL
https://repisalud.isciii.es/bitstreams/9f86ffcb-e279-4f7a-8433-4c97854f4dc3/download
File
MD5
b3f15a3ff9abe9c3f484ce3ee8e37a5b
103797
application/pdf
SystemsBiologyOfTissue-Specific_2015_S4_Table.pdf
URL
https://repisalud.isciii.es/bitstreams/4cefe817-5b20-48a6-afd9-9af4a6f4f511/download
File
MD5
b8ea8ad2b61fa7578624dc7bbff8ccff
3007308
image/tiff
SystemsBiologyOfTissue-Specific_2015_S5_Fig.tif
URL
https://repisalud.isciii.es/bitstreams/0361d4db-d6fb-4982-a7d1-7d303cccb9c7/download
File
MD5
590779f80cf9cffb146aa37aad43f267
57054
application/pdf
SystemsBiologyOfTissue-Specific_2015_S5_Table.pdf
URL
https://repisalud.isciii.es/bitstreams/09dec4d0-4fc6-4f07-9f81-9f3695957f99/download
File
MD5
d9e81b3ca1ea3f6b4f169d38f04839c6
3007854
image/tiff
SystemsBiologyOfTissue-Specific_2015_S6_Fig.tif
URL
https://repisalud.isciii.es/bitstreams/20e76801-ef76-4b58-a4d8-596a3f0b8c34/download
File
MD5
94d93b5d1654675e81906003c6f15c10
95323
text/plain
SystemsBiologyOfTissue-Specific_2015.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/528219a9-a4e3-457f-ae98-cbf58528b76b/download
File
MD5
793a4e6a9d7dd5e5ac88169b70253766
686
text/plain
SystemsBiologyOfTissue-Specific_2015_S1_Table.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/1c9ae9f1-2a87-45d0-a9dc-577d0cb168a5/download
File
MD5
43c1dc507bee000b6146d96d8d29e691
6350
text/plain
SystemsBiologyOfTissue-Specific_2015_S4_Table.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/c0f00cbb-3cca-4922-82c2-5429cc24de10/download
File
MD5
189987470e1af70d914f4da3fb311ff8
1767
text/plain
SystemsBiologyOfTissue-Specific_2015_S5_Table.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/dc743b2d-35dd-46be-b7b9-8398b8aa1972/download
File
MD5
230ae2ef3c4130c8c1ac8ad4679cb309
100000
text/plain
SystemsBiologyOfTissue-Specific_2015_S2_Table.xlsx.txt
URL
https://repisalud.isciii.es/bitstreams/84bfbdc6-ebac-45b7-8575-9092ed33ef88/download
File
MD5
19558c1f261e317f28c0916fe1fc6973
100000
text/plain
SystemsBiologyOfTissue-Specific_2015_S3_Table.xlsx.txt