2026-08-10T07:23:55Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/54092024-09-27T09:17:10Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Leskela, Susanna
author
Serrano, Ana
author
de la Fuente, Hortensia
author
Rodriguez-Munoz, Ana
author
Ramos-Levi, Ana
author
Sampedro-Nuñez, Miguel
author
Sanchez-Madrid, Francisco
author
Gonzalez-Amaro, Roberto
author
Marazuela, Monica
funder
Instituto de Salud Carlos III
funder
Fondo de Cooperación Internacional en Ciencia y Tecnología Unión Europea-Mexico
funder
Comunidad de Madrid (España)
2017-11-27T13:49:52Z
2017-11-27T13:49:52Z
2015
PLoS One. 2015; 10(4):e0123938
10.1371/journal.pone.0123938
1932-6203
PLoS One
25880730
http://hdl.handle.net/20.500.12105/5409
Introduction
Patients with autoimmune thyroid disease (AITD) show defects in their
immune-regulatory mechanisms. Herein we assessed the expression and
function of galectin-1 and galectin-9 (Gal-1, Gal-9) in dendritic cells
(DCs) from patients with AITD.
Materials and Methods
Peripheral blood samples from 25 patients with Graves' disease (GD), 11
Hashimoto's thyroiditis (HT), and 24 healthy subjects were studied.
Thyroid tissue samples from 44 patients with AITD and 22 patients with
goiter were also analyzed. Expression and function of Gal-1 and Gal-9
was assessed by quantitative RT-PCR, immunofluorescence and flow
cytometry.
Results
A diminished expression of Gal-9, but not of Gal-1, by peripheral blood
DCs was observed in GD patients, mainly in those with Graves `
ophthalmopathy, and a significant negative association between disease
severity and Gal-9 expression was detected. In addition, the mRNA levels
of Gal-9 and its ligand TIM-3 were increased in thyroid tissue from AITD
patients and its expression was associated with the levels of
Th1/Th12/Th17 cytokines. Immunofluorescence studies proved that
intrathyroidal Gal-9 expression was confined to DCs and macrophages.
Finally, in vitro functional assays showed that exogenous Gal-9 had a
suppressive effect on the release of Th1/Th2/Th17 cytokines by
DC/lymphocyte autologous co-cultures from both AITD patients and healthy
controls.
Conclusions
The altered pattern of expression of Gal-9 in peripheral blood DCs from
GD patients, its correlation with disease severity as well as its
ability to suppress cytokine release suggest that Gal-9 could be
involved in the pathogenesis of AITD.
eng
AUTOIMMUNE THYROID-DISEASE
T-CELLS
OPHTHALMOPATHY
PROLIFERATION
GLYCOSYLATION
INFLAMMATION
LYMPHOCYTES
SUPPRESSES
ACTIVATION
INDUCTION
Graves' Disease Is Associated with a Defective Expression of the Immune
Regulatory Molecule Galectin-9 in Antigen-Presenting Dendritic Cells
journal article
URL
https://repisalud.isciii.es/bitstreams/63019e89-7fd7-4e38-a10f-62cb083c1761/download
File
MD5
6bce59e98bd18b8283d5bf73e305db1e
3055994
application/pdf
GravesDiseaseIsAssociated_2015.pdf
URL
https://repisalud.isciii.es/bitstreams/a90f5019-8345-4a32-a3b2-eb907f25faca/download
File
MD5
d57bbfd7b7515d6469cb43ad0c8a8891
1275736
image/tiff
GravesDiseaseIsAssociated_2015_S1_Fig.tif
URL
https://repisalud.isciii.es/bitstreams/b72599a8-1088-4caa-a182-f78687fb8271/download
File
MD5
3ec67048ada123d16851be79186429fb
81920
application/msword
GravesDiseaseIsAssociated_2015_S1_Table.doc
URL
https://repisalud.isciii.es/bitstreams/fd5f89dc-7ca3-439b-b46f-28587298d5e0/download
File
MD5
4396ec2bc5efc297efb8716a91417392
2400738
image/tiff
GravesDiseaseIsAssociated_2015_S2_Fig.tif
URL
https://repisalud.isciii.es/bitstreams/bc2d9710-99d0-418b-8bde-8ea8ed4b6d01/download
File
MD5
745edaf8fa3fcee65ed534c3ffb7379d
36864
application/msword
GravesDiseaseIsAssociated_2015_S2_Table.doc
URL
https://repisalud.isciii.es/bitstreams/eaf0138f-9bc8-42e4-8502-6e8adc6b7635/download
File
MD5
424e50067cf8ac35b0811404c5e2bc27
2826470
image/tiff
GravesDiseaseIsAssociated_2015_S3_Fig.tif
URL
https://repisalud.isciii.es/bitstreams/4838590f-2e01-407b-a37f-fb48bb1e1731/download
File
MD5
de02ca374ef4ffae4d02565ec3dbd213
40448
application/msword
GravesDiseaseIsAssociated_2015_S3_Table.doc
URL
https://repisalud.isciii.es/bitstreams/a3353178-bc62-4b5b-b4f1-1d341daca8a3/download
File
MD5
975ae8fc1deb58f995a3b6d080b50215
979808
image/tiff
GravesDiseaseIsAssociated_2015_S4_Fig.tif
URL
https://repisalud.isciii.es/bitstreams/00f6393d-5525-4919-8fd2-a16b45d3334e/download
File
MD5
1ba7c18e883c731dde896562b91be976
46691
text/plain
GravesDiseaseIsAssociated_2015.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/a42e7c2b-d909-4174-b548-0d6c50cdff2e/download
File
MD5
60bf95175f4f1139d73cc5295b0f348f
1667
text/plain
GravesDiseaseIsAssociated_2015_S1_Table.doc.txt
URL
https://repisalud.isciii.es/bitstreams/0b3c2781-a163-45a4-9c64-2ec49f86b04f/download
File
MD5
233f1762ef6463a58e9f1e88c1450ca8
548
text/plain
GravesDiseaseIsAssociated_2015_S2_Table.doc.txt
URL
https://repisalud.isciii.es/bitstreams/e98759be-c494-4618-ad10-a63a124025a8/download
File
MD5
855240fa706cf191f3256f8903698b7a
1279
text/plain
GravesDiseaseIsAssociated_2015_S3_Table.doc.txt