2026-07-21T14:00:37Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/54062024-11-29T12:17:11Zcom_20.500.12105_19604com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19605col_20.500.12105_16988
Repisalud
author
Soto, Manuel
author
Corvo, Laura
author
Garde, Esther
author
Ramirez, Laura
author
Iniesta, Virginia
author
Bonay, Pedro
author
Gomez-Nieto, Carlos
author
González, Victor M
author
Elena Martin, M.
author
Alonso, Carlos
author
Coelho, Eduardo A. F.
author
Barral, Aldina
author
Barral-Netto, Manoel
author
Iborra, Salvador
funder
Ministerio de Ciencia e Innovación (España)
funder
RETICS-Investigación colaborativa en Enfermedades Tropicales (RICET-ISCIII) (España)
funder
Instituto de Salud Carlos III
funder
Fundación Ramón Areces
funder
National Council for Scientific and Technological Development (Brasil)
2017-11-27T13:49:52Z
2017-11-27T13:49:52Z
2015
PLoS Negl Trop Dis. 2015; 9(5):e0003751
10.1371/journal.pntd.0003751
1935-2735
PLoS Neglected Tropical Diseases
25955652
http://hdl.handle.net/20.500.12105/5406
Background: Highly conserved intracellular proteins from Leishmania have been described as antigens in natural and experimental infected mammals. The present study aimed to evaluate the antigenicity and prophylactic properties of the Leishmania infantum Poly (A) binding proteins (LiPABPs). Methodology/Principal Findings: Three different members of the LiPABP family have been described. Recombinant tools based on these proteins were constructed: recombinant proteins and DNA vaccines. The three recombinant proteins were employed for coating ELISA plates. Sera from human and canine patients of visceral leishmaniasis and human patients of mucosal leishmaniasis recognized the three LiPABPs. In addition, the protective efficacy of a DNA vaccine based on the combination of the three Leishmania PABPs has been tested in a model of progressive murine leishmaniasis: BALB/c mice infected with Leishmania major. The induction of a Th1-like response against the LiPABP family by genetic vaccination was able to down-regulate the IL-10 predominant responses elicited by parasite LiPABPs after infection in this murine model. This modulation resulted in a partial protection against L. major infection. LiPABP vaccinated mice showed a reduction on the pathology that was accompanied by a decrease in parasite burdens, in antibody titers against Leishmania antigens and in the IL-4 and IL-10 parasite-specific mediated responses in comparison to control mice groups immunized with saline or with the non-recombinant plasmid. Conclusion/Significance: The results presented here demonstrate for the first time the prophylactic properties of a new family of Leishmania antigenic intracellular proteins, the LiPABPs. The redirection of the immune response elicited against the LiPABP family (from IL-10 towards IFN-gamma mediated responses) by genetic vaccination was able to induce a partial protection against the development of the disease in a highly susceptible murine model of leishmaniasis.
eng
CANINE VISCERAL LEISHMANIASIS
PRIME-BOOST VACCINATION
CUTANEOUS
LEISHMANIASIS
T-CELL
IMMUNE-RESPONSES
VIANNIA BRAZILIENSIS
EXPRESSION CLONING
CONFERS PROTECTION
INCLUSION-BODIES
HISTONE H2B
Coadministration of the Three Antigenic Leishmania infantum Poly (A)
Binding Proteins as a DNA Vaccine Induces Protection against Leishmania
major Infection in BALB/c Mice
journal article
URL
https://repisalud.isciii.es/bitstreams/3c9c3615-91a7-4d30-8ceb-8bc26788fce1/download
File
MD5
4884277c00a6ff25add1f63f90df632b
2095188
application/pdf
CoadministrationOfTheThreeAntigenic_2015.pdf
URL
https://repisalud.isciii.es/bitstreams/550a4372-c2b7-4518-88d8-8b921b3fb06a/download
File
MD5
83cb921469e774ba92e0c68053c9aaa0
87106
text/plain
CoadministrationOfTheThreeAntigenic_2015.pdf.txt