2026-07-21T14:00:36Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/54062024-11-29T12:17:11Zcom_20.500.12105_19604com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19605col_20.500.12105_16988

00925njm 22002777a 4500 dc Soto, Manuel author Corvo, Laura author Garde, Esther author Ramirez, Laura author Iniesta, Virginia author Bonay, Pedro author Gomez-Nieto, Carlos author González, Victor M author Elena Martin, M. author Alonso, Carlos author Coelho, Eduardo A. F. author Barral, Aldina author Barral-Netto, Manoel author Iborra, Salvador author 2015 Background: Highly conserved intracellular proteins from Leishmania have been described as antigens in natural and experimental infected mammals. The present study aimed to evaluate the antigenicity and prophylactic properties of the Leishmania infantum Poly (A) binding proteins (LiPABPs). Methodology/Principal Findings: Three different members of the LiPABP family have been described. Recombinant tools based on these proteins were constructed: recombinant proteins and DNA vaccines. The three recombinant proteins were employed for coating ELISA plates. Sera from human and canine patients of visceral leishmaniasis and human patients of mucosal leishmaniasis recognized the three LiPABPs. In addition, the protective efficacy of a DNA vaccine based on the combination of the three Leishmania PABPs has been tested in a model of progressive murine leishmaniasis: BALB/c mice infected with Leishmania major. The induction of a Th1-like response against the LiPABP family by genetic vaccination was able to down-regulate the IL-10 predominant responses elicited by parasite LiPABPs after infection in this murine model. This modulation resulted in a partial protection against L. major infection. LiPABP vaccinated mice showed a reduction on the pathology that was accompanied by a decrease in parasite burdens, in antibody titers against Leishmania antigens and in the IL-4 and IL-10 parasite-specific mediated responses in comparison to control mice groups immunized with saline or with the non-recombinant plasmid. Conclusion/Significance: The results presented here demonstrate for the first time the prophylactic properties of a new family of Leishmania antigenic intracellular proteins, the LiPABPs. The redirection of the immune response elicited against the LiPABP family (from IL-10 towards IFN-gamma mediated responses) by genetic vaccination was able to induce a partial protection against the development of the disease in a highly susceptible murine model of leishmaniasis. PLoS Negl Trop Dis. 2015; 9(5):e0003751 10.1371/journal.pntd.0003751 1935-2735 PLoS Neglected Tropical Diseases 25955652 http://hdl.handle.net/20.500.12105/5406 CANINE VISCERAL LEISHMANIASIS PRIME-BOOST VACCINATION CUTANEOUS LEISHMANIASIS T-CELL IMMUNE-RESPONSES VIANNIA BRAZILIENSIS EXPRESSION CLONING CONFERS PROTECTION INCLUSION-BODIES HISTONE H2B Coadministration of the Three Antigenic Leishmania infantum Poly (A) Binding Proteins as a DNA Vaccine Induces Protection against Leishmania major Infection in BALB/c Mice