2026-04-29T22:57:36Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/54012024-09-27T09:00:26Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607
Repisalud
author
Otero-Ortega, Laura
author
Gutierrez-Fernandez, Maria
author
Ramos-Cejudo, Jaime
author
Rodriguez-Frutos, Berta
author
Fuentes, Blanca
author
Sobrino, Tomas
author
Navarro Hernanz, Teresa
author
Campos, Francisco
author
Lopez, Juan Antonio
author
Cerdan, Sebastian
author
Vazquez, Jesus
author
Diez-Tejedor, Exuperio
funder
Ministerio de Economía y Competitividad (España)
funder
Instituto de Salud Carlos III
2017-11-27T13:49:51Z
2017-11-27T13:49:51Z
2015
Stem Cell Res Ther. 2015; 6(1):121
10.1186/s13287-015-0111-4
1757-6512
Stem Cell Research & Therapy
26088123
http://hdl.handle.net/20.500.12105/5401
Introduction: Despite its high incidence, nerve fiber (axon and myelin)
damage after cerebral infarct has not yet been extensively investigated.
The aim of this study was to investigate white matter repair after
adipose-derived mesenchymal stem cell (ADMSC) administration in an
experimental model of subcortical stroke. Furthermore, we aimed to
analyze the ADMSC secretome and whether this could be implicated in this
repair function.
Methods: An animal model of subcortical ischemic stroke with white
matter affectation was induced in rats by injection of endothelin-1. At
24 hours, 2 x 10(6) ADMSC were administered intravenously to the
treatment group. Functional evaluation, lesion size, fiber tract
integrity, cell death, proliferation, white matter repair markers
(Olig-2, NF, and MBP) and NogoA were all studied after sacrifice (7 days
and 28 days). ADMSC migration and implantation in the brain as well as
proteom cs analysis and functions of the secretome were also analyzed.
Results: Neither ADMSC migration nor implantation to the brain was
observed after ADMSC administration. In contrast, ADMSC implantation was
detected in peripheral organs. The treatment group showed a smaller
functional deficit, smaller lesion area, less cell death, more
oligodendrocyte proliferation, more white matter connectivity and higher
amounts of myelin formation. The treated animals also showed higher
levels of white matter-associated markers in the injured area than the
control group. Proteomics analysis of the ADMSC secretome identified
2,416 proteins, not all of them previously described to be involved in
brain plasticity.
Conclusions: White matter integrity in subcortical stroke is in part
restored by ADMSC treatment; this is mediated by repair molecular
factors implicated in axonal sprouting, remyelination and
oligodendrogenesis. These findings are associated with improved
functional recovery after stroke.
eng
BONE-MARROW
BRAIN
HEMORRHAGE
DELIVERY
MODEL
RAT
White matter injury restoration after stem cell administration in
subcortical ischemic stroke
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