2026-04-29T22:57:34Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/54012024-09-27T09:00:26Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607

00925njm 22002777a 4500 dc Otero-Ortega, Laura author Gutierrez-Fernandez, Maria author Ramos-Cejudo, Jaime author Rodriguez-Frutos, Berta author Fuentes, Blanca author Sobrino, Tomas author Navarro Hernanz, Teresa author Campos, Francisco author Lopez, Juan Antonio author Cerdan, Sebastian author Vazquez, Jesus author Diez-Tejedor, Exuperio author 2015 Introduction: Despite its high incidence, nerve fiber (axon and myelin) damage after cerebral infarct has not yet been extensively investigated. The aim of this study was to investigate white matter repair after adipose-derived mesenchymal stem cell (ADMSC) administration in an experimental model of subcortical stroke. Furthermore, we aimed to analyze the ADMSC secretome and whether this could be implicated in this repair function. Methods: An animal model of subcortical ischemic stroke with white matter affectation was induced in rats by injection of endothelin-1. At 24 hours, 2 x 10(6) ADMSC were administered intravenously to the treatment group. Functional evaluation, lesion size, fiber tract integrity, cell death, proliferation, white matter repair markers (Olig-2, NF, and MBP) and NogoA were all studied after sacrifice (7 days and 28 days). ADMSC migration and implantation in the brain as well as proteom cs analysis and functions of the secretome were also analyzed. Results: Neither ADMSC migration nor implantation to the brain was observed after ADMSC administration. In contrast, ADMSC implantation was detected in peripheral organs. The treatment group showed a smaller functional deficit, smaller lesion area, less cell death, more oligodendrocyte proliferation, more white matter connectivity and higher amounts of myelin formation. The treated animals also showed higher levels of white matter-associated markers in the injured area than the control group. Proteomics analysis of the ADMSC secretome identified 2,416 proteins, not all of them previously described to be involved in brain plasticity. Conclusions: White matter integrity in subcortical stroke is in part restored by ADMSC treatment; this is mediated by repair molecular factors implicated in axonal sprouting, remyelination and oligodendrogenesis. These findings are associated with improved functional recovery after stroke. Stem Cell Res Ther. 2015; 6(1):121 10.1186/s13287-015-0111-4 1757-6512 Stem Cell Research & Therapy 26088123 http://hdl.handle.net/20.500.12105/5401 BONE-MARROW BRAIN HEMORRHAGE DELIVERY MODEL RAT White matter injury restoration after stem cell administration in subcortical ischemic stroke