2026-08-29T14:55:11Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/53962024-11-29T08:32:42Zcom_20.500.12105_19604com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19605col_20.500.12105_16969
Repisalud
author
Tornero-Esteban, Pilar
author
Rodriguez-Rodriguez, Luis
author
Abasolo, Lydia
author
Tome, Maria
author
Lopez-Romero, Pedro
author
Herranz, Eva
author
Gomez, Manuel J
author
Marco, Fernando
author
Moro, Enrique
author
Fernandez-Gutierrez, Benjamin
author
Ramon Lamas, Jose
funder
Instituto de Salud Carlos III
funder
Sociedad Española de Cirugía Ortopédica y Traumatología
2017-11-27T13:49:50Z
2017-11-27T13:49:50Z
2015
BMC Musculoskelet Disord. 2015; 16:182
10.1186/s12891-015-0652-9
1471-2474
BMC Musculoskeletal Disorders
26243143
http://hdl.handle.net/20.500.12105/5396
Background: The aim of this study was to evaluate, the existence of a
signature of differentially expressed microRNAs (miRNAs) during
osteogenic differentiation of bone marrow MSCs from OA and healthy
donors and to describe their possible implication in joint regeneration
through modulation of molecular mechanisms involved in homeostatic
control in OA pathophysiology.
Methods: Following phenotypic assessment of BM-MSCs obtained from OA
diagnosed patients (n = 10) and non-OA (n = 10), total small RNA was
isolated after osteogenic induction for 1, 10 and 21 days, miRNA
profiles were generated using a commercial expression array of 754
well-characterized miRNAs. MiRNAs, with consistent differential
expression were selected for further validation by quantitative
reverse-transcription polymerase chain reaction (qRT-PCR) analysis.
Results: A total of 246 miRNAs were differentially expressed (fold
change >=+/- 2, P <= 0.05) between OA and non-OA BM-MSC samples; these
miRNAs showed variable interactions depending on the cell and
differentiation status. Two miRNAs, hsa-miR-210 and hsa-miR-335-5p out
of 21 used for validation showed a significant downregulated expression
during induced osteogenesis. In particular hsa-miR-335-5p, a critical
regulator in bone homeostasis, was further studied. hsa-miR-335-5p
downregulation in OA-MSCs, as well as their host coding gene, MEST, were
also assessed.
Conclusions: To our knowledge, this study represents the most
comprehensive assessment to date of miRNA expression profiling in
BM-MSCs from OA patients and their role during osteogenic
differentiation. We describe the existence of a correlation between
miR-335-5p expression and OA indicating the putative role of this miRNA
in OA features. These findings, may contribute to our understanding of
the molecular mechanisms involved in MSCs mediated homeostatic control
in OA pathophysiology that could be applicable in future therapeutic
approaches.
eng
MESENCHYMAL STEM-CELLS
GENE-EXPRESSION
BETA-CATENIN
ENDOCHONDRAL
OSSIFICATION
ARTICULAR-CARTILAGE
SIGNALING PATHWAY
WNT
CHONDROGENESIS
PATHOGENESIS
DEGRADATION
Signature of microRNA expression during osteogenic differentiation of
bone marrow MSCs reveals a putative role of miR-335-5p in osteoarthritis
journal article
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