2026-08-29T14:55:33Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/53962024-11-29T08:32:42Zcom_20.500.12105_19604com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19605col_20.500.12105_16969

00925njm 22002777a 4500 dc Tornero-Esteban, Pilar author Rodriguez-Rodriguez, Luis author Abasolo, Lydia author Tome, Maria author Lopez-Romero, Pedro author Herranz, Eva author Gomez, Manuel J author Marco, Fernando author Moro, Enrique author Fernandez-Gutierrez, Benjamin author Ramon Lamas, Jose author 2015 Background: The aim of this study was to evaluate, the existence of a signature of differentially expressed microRNAs (miRNAs) during osteogenic differentiation of bone marrow MSCs from OA and healthy donors and to describe their possible implication in joint regeneration through modulation of molecular mechanisms involved in homeostatic control in OA pathophysiology. Methods: Following phenotypic assessment of BM-MSCs obtained from OA diagnosed patients (n = 10) and non-OA (n = 10), total small RNA was isolated after osteogenic induction for 1, 10 and 21 days, miRNA profiles were generated using a commercial expression array of 754 well-characterized miRNAs. MiRNAs, with consistent differential expression were selected for further validation by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis. Results: A total of 246 miRNAs were differentially expressed (fold change >=+/- 2, P <= 0.05) between OA and non-OA BM-MSC samples; these miRNAs showed variable interactions depending on the cell and differentiation status. Two miRNAs, hsa-miR-210 and hsa-miR-335-5p out of 21 used for validation showed a significant downregulated expression during induced osteogenesis. In particular hsa-miR-335-5p, a critical regulator in bone homeostasis, was further studied. hsa-miR-335-5p downregulation in OA-MSCs, as well as their host coding gene, MEST, were also assessed. Conclusions: To our knowledge, this study represents the most comprehensive assessment to date of miRNA expression profiling in BM-MSCs from OA patients and their role during osteogenic differentiation. We describe the existence of a correlation between miR-335-5p expression and OA indicating the putative role of this miRNA in OA features. These findings, may contribute to our understanding of the molecular mechanisms involved in MSCs mediated homeostatic control in OA pathophysiology that could be applicable in future therapeutic approaches. BMC Musculoskelet Disord. 2015; 16:182 10.1186/s12891-015-0652-9 1471-2474 BMC Musculoskeletal Disorders 26243143 http://hdl.handle.net/20.500.12105/5396 MESENCHYMAL STEM-CELLS GENE-EXPRESSION BETA-CATENIN ENDOCHONDRAL OSSIFICATION ARTICULAR-CARTILAGE SIGNALING PATHWAY WNT CHONDROGENESIS PATHOGENESIS DEGRADATION Signature of microRNA expression during osteogenic differentiation of bone marrow MSCs reveals a putative role of miR-335-5p in osteoarthritis