2026-08-29T22:04:22Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/53932024-11-30T01:11:11Zcom_20.500.12105_2173com_20.500.12105_2051com_20.500.12105_19604col_20.500.12105_19597col_20.500.12105_19607
Repisalud
author
Torres-Ruiz, Raul
author
Rodriguez Perales, Sandra
funder
Instituto de Salud Carlos III
2017-11-27T13:49:50Z
2017-11-27T13:49:50Z
2015
Int J Mol Sci. 2015; 16(9):22151-68
10.3390/ijms160922151
1422-0067
International Journal of Molecular Sciences
26389881
http://hdl.handle.net/20.500.12105/5393
The cancer-modelling field is now experiencing a conversion with the
recent emergence of the RNA-programmable CRISPR-Cas9 system, a flexible
methodology to produce essentially any desired modification in the
genome. Cancer is a multistep process that involves many genetic
mutations and other genome rearrangements. Despite their importance, it
is difficult to recapitulate the degree of genetic complexity found in
patient tumors. The CRISPR-Cas9 system for genome editing has been
proven as a robust technology that makes it possible to generate
cellular and animal models that recapitulate those cooperative
alterations rapidly and at low cost. In this review, we will discuss the
innovative applications of the CRISPR-Cas9 system to generate new
models, providing a new way to interrogate the development and
progression of cancers.
eng
CRISPR-Cas9 system
Cancer modelling
Gene mutations
Rearrangements
Genome engineering
ZINC-FINGER NUCLEASES
OFF-TARGET MUTATIONS
ONE-STEP GENERATION
IN-VIVO
HUMAN-CELLS
HUMAN GENOME
CHROMOSOMAL REARRANGEMENTS
CAS9 RIBONUCLEOPROTEINS
TUMOR-SUPPRESSOR
MAMMALIAN-CELLS
CRISPR-Cas9: A Revolutionary Tool for Cancer Modelling
journal article
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CRISPR-Cas9ARevolutionaryTool_2015.pdf
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CRISPR-Cas9ARevolutionaryTool_2015.pdf.txt