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00925njm 22002777a 4500 dc Torres-Ruiz, Raul author Rodriguez Perales, Sandra author 2015 The cancer-modelling field is now experiencing a conversion with the recent emergence of the RNA-programmable CRISPR-Cas9 system, a flexible methodology to produce essentially any desired modification in the genome. Cancer is a multistep process that involves many genetic mutations and other genome rearrangements. Despite their importance, it is difficult to recapitulate the degree of genetic complexity found in patient tumors. The CRISPR-Cas9 system for genome editing has been proven as a robust technology that makes it possible to generate cellular and animal models that recapitulate those cooperative alterations rapidly and at low cost. In this review, we will discuss the innovative applications of the CRISPR-Cas9 system to generate new models, providing a new way to interrogate the development and progression of cancers. Int J Mol Sci. 2015; 16(9):22151-68 10.3390/ijms160922151 1422-0067 International Journal of Molecular Sciences 26389881 http://hdl.handle.net/20.500.12105/5393 CRISPR-Cas9 system Cancer modelling Gene mutations Rearrangements Genome engineering ZINC-FINGER NUCLEASES OFF-TARGET MUTATIONS ONE-STEP GENERATION IN-VIVO HUMAN-CELLS HUMAN GENOME CHROMOSOMAL REARRANGEMENTS CAS9 RIBONUCLEOPROTEINS TUMOR-SUPPRESSOR MAMMALIAN-CELLS CRISPR-Cas9: A Revolutionary Tool for Cancer Modelling