2026-05-21T23:28:12Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/53922024-10-31T11:40:01Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Villar, Margarita
author
Ayllon, Nieves
author
Kocan, Katherine M.
author
Bonzon-Kulichenko, Elena
author
Alberdi, Pilar
author
Blouin, Edmour F.
author
Weisheit, Sabine
author
Mateos-Hernandez, Lourdes
author
Cabezas-Cruz, Alejandro
author
Bell-Sakyi, Lesley
author
Vancova, Marie
author
Bily, Tomas
author
Meyer, Damien F.
author
Sterba, Jan
author
Contreras, Marinela
author
Rudenko, Nataliia
author
Grubhoffer, Libor
author
Vazquez, Jesus
author
de la Fuente, Jose
funder
Unión Europea
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Ministerio de Educación, Cultura y Deporte (España)
2017-11-27T13:49:50Z
2017-11-27T13:49:50Z
2015
PLoS One. 2015; 10(9):e0137237
10.1371/journal.pone.0137237
1932-6203
PLoS One
26340562
http://hdl.handle.net/20.500.12105/5392
Anaplasma phagocytophilum is an emerging zoonotic pathogen transmitted
by Ixodes scapularis that causes human granulocytic anaplasmosis. Here,
a high throughput quantitative proteomics approach was used to
characterize A. phagocytophilum proteome during rickettsial
multiplication and identify proteins involved in infection of the tick
vector, I. scapularis. The first step in this research was focused on
tick cells infected with A. phagocytophilum and sampled at two time
points containing 10-15\% and 65-71\% infected cells, respectively to
identify key bacterial proteins over-represented in high percentage
infected cells. The second step was focused on adult female tick guts
and salivary glands infected with A. phagocytophilum to compare in vitro
results with those occurring during bacterial infection in vivo. The
results showed differences in the proteome of A. phagocytophilum in
infected ticks with higher impact on protein synthesis and processing
than on bacterial replication in tick salivary glands. These results
correlated well with the developmental cycle of A. phagocytophilum, in
which cells convert from an intracellular reticulated, replicative form
to the nondividing infectious dense-core form. The analysis of A.
phagocytophilum differentially represented proteins identified stress
response (GroEL, HSP70) and surface (MSP4) proteins that were
over-represented in high percentage infected tick cells and salivary
glands when compared to low percentage infected cells and guts,
respectively. The results demonstrated that MSP4, GroEL and HSP70
interact and bind to tick cells, thus playing a role in rickettsia-tick
interactions. The most important finding of these studies is the
increase in the level of certain bacterial stress response and surface
proteins in A. phagocytophilum-infected tick cells and salivary glands
with functional implication in tick-pathogen interactions. These results
gave a new dimension to the role of these stress response and surface
proteins during A. phagocytophilum infection in ticks. Characterization
of Anaplasma proteome contributes information on host-pathogen
interactions and provides targets for development of novel control
strategies for pathogen infection and transmission.
eng
IV SECRETION SYSTEM
OUTER-MEMBRANE VESICLES
GENE-EXPRESSION
SURFACE-PROTEINS
DIFFERENTIAL EXPRESSION
CATTLE PATHOGEN
MARGINALE
CELLS
ADHESION
STRESS
Identification and Characterization of Anaplasma phagocytophilum
Proteins Involved in Infection of the Tick Vector, Ixodes scapularis
journal article
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IdentificationandCharacterizationofAnaplasma_2015.pdf
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