2026-04-29T07:21:39Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/53842024-09-27T09:36:27Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Cruz, Francisco Miguel
author
Tome, Maria
author
Bernal, Juan Antonio
author
Bernad, Antonio
funder
Ministerio de Ciencia e Innovación (España)
funder
Comunidad de Madrid (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Comisión Europea
2017-11-27T13:49:48Z
2017-11-27T13:49:48Z
2015
Cell Death Dis. 2015; 6:e1953
2041-4889
http://hdl.handle.net/20.500.12105/5384
26512961
10.1038/cddis.2015.255
Cell Death & Differentiation
B lymphoma Mo-MLV insertion region 1 (Bmi1) is a polycomb-family
transcriptional factor critical for self-renewal in many adult stem
cells and human neoplasia. We sought to identify microRNAs regulated by
Bmi1 that could play a role in multipotent cardiac progenitor cell (CPC)
decisions. We found that miR-300, a poorly characterized microRNA
mapping in the Dlk1-Dio3 microRNA cluster, was positively regulated by
Bmi1 in CPCs. Forced expression of miR-300 in CPCs promoted an improved
stemness signature with a significant increase in Oct4 levels, a
reduction in senescence progression and an enhanced proliferative status
via p19 activation and inhibition of p16 accumulation. Endothelial and
cardiogenic differentiation were clearly compromised by sustained
miR-300 expression. Additionally, RNA and protein analysis revealed a
significant reduction in key cardiac transcription factors, including
Nkx2.5 and Tbx5. Collectively, these results suggest that some functions
attributed to Bmi1 are due to induction of miR-300, which decreases the
cardiogenic differentiation potential of multipotent CPCs in vitro and
promotes self-renewal.
eng
CELL SELF-RENEWAL
RANDOMIZED PHASE-1 TRIAL
ACTIVATED PROTEIN-KINASE
INTESTINAL STEM-CELLS
MYOCARDIAL-INFARCTION
HEART
PATHWAY
CANCER
CARDIOMYOPATHY
CARDIOMYOCYTES
miR-300 mediates Bmi1 function and regulates differentiation in
primitive cardiac progenitors
journal article
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MiR-300MediatesBmi1Function_2015.pdf
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MiR-300MediatesBmi1Function_2015.pdf.txt