2026-08-05T15:42:47Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/52592024-09-27T09:10:18Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607
Repisalud
author
Ezkurdia, Iakes
author
Calvo, Enrique
author
Del Pozo, Angela
author
Vazquez, Jesus
author
Valencia, Alfonso
author
Tress, Michael L.
funder
National Institutes of Health (Estados Unidos)
funder
Ministerio de Economía y Competitividad (España)
2017-10-30T13:32:28Z
2017-10-30T13:32:28Z
2015
Expert Rev Proteomics. 2015; 12(6):579-93
10.1586/14789450.2015.1103186
1744-8387
1478-9450
Expert Review of Proteomics
26496066
http://hdl.handle.net/20.500.12105/5259
The authors have carried out an investigation of the two draft maps of the human proteome published in 2014 in Nature. The findings include an abundance of poor spectra, low-scoring peptide-spectrum matches and incorrectly identified proteins in both these studies, highlighting clear issues with the application of false discovery rates. This noise means that the claims made by the two papers - the identification of high numbers of protein coding genes, the detection of novel coding regions and the draft tissue maps themselves - should be treated with considerable caution. The authors recommend that clinicians and researchers do not use the unfiltered data from these studies. Despite this these studies will inspire further investigation into tissue-based proteomics. As long as this future work has proper quality controls, it could help produce a consensus map of the human proteome and improve our understanding of the processes that underlie health and disease.
eng
Clinical applications
false discovery rates
human proteome
protein coding genes
proteomics
FALSE DISCOVERY RATE
SORF-ENCODED POLYPEPTIDES
MASS-SPECTROMETRY
MISSING PROTEINS
CODING GENES
HUMAN GENOME
IDENTIFICATION
PROJECT
DATABASE
PEPTIDE
The potential clinical impact of the release of two drafts of the human proteome
journal article
URL
https://repisalud.isciii.es/bitstreams/b056875e-8c5f-45e3-9af8-2a775f764ca2/download
File
MD5
29e57b3c2d44f51bd39d5cf26a2c8489
1741845
application/pdf
ThePotentialClinicalImpact_2015.pdf
URL
https://repisalud.isciii.es/bitstreams/514e5774-feef-49d6-8649-7a15baf84ec4/download
File
MD5
1d5603eab27684bf45dcf201261c893a
75044
text/plain
ThePotentialClinicalImpact_2015.pdf.txt