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oai:repisalud.isciii.es:20.500.12105/52592024-09-27T09:10:18Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607

00925njm 22002777a 4500 dc Ezkurdia, Iakes author Calvo, Enrique author Del Pozo, Angela author Vazquez, Jesus author Valencia, Alfonso author Tress, Michael L. author 2015 The authors have carried out an investigation of the two draft maps of the human proteome published in 2014 in Nature. The findings include an abundance of poor spectra, low-scoring peptide-spectrum matches and incorrectly identified proteins in both these studies, highlighting clear issues with the application of false discovery rates. This noise means that the claims made by the two papers - the identification of high numbers of protein coding genes, the detection of novel coding regions and the draft tissue maps themselves - should be treated with considerable caution. The authors recommend that clinicians and researchers do not use the unfiltered data from these studies. Despite this these studies will inspire further investigation into tissue-based proteomics. As long as this future work has proper quality controls, it could help produce a consensus map of the human proteome and improve our understanding of the processes that underlie health and disease. Expert Rev Proteomics. 2015; 12(6):579-93 10.1586/14789450.2015.1103186 1744-8387 1478-9450 Expert Review of Proteomics 26496066 http://hdl.handle.net/20.500.12105/5259 Clinical applications false discovery rates human proteome protein coding genes proteomics FALSE DISCOVERY RATE SORF-ENCODED POLYPEPTIDES MASS-SPECTROMETRY MISSING PROTEINS CODING GENES HUMAN GENOME IDENTIFICATION PROJECT DATABASE PEPTIDE The potential clinical impact of the release of two drafts of the human proteome