2026-06-14T03:37:42Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/52412025-02-27T11:24:52Zcom_20.500.12105_19604com_20.500.12105_2051com_20.500.12105_2173col_20.500.12105_19605col_20.500.12105_19597col_20.500.12105_19607
Repisalud
author
Blas-Rus, Noelia
author
Bustos-Moran, Eugenio
author
Perez de Castro, Ignacio
author
de Carcer Diez, Guillermo
author
Borroto, Aldo
author
Camafeita, Emilio
author
Jorge, Inmaculada
author
Vazquez, Jesus
author
Alarcón, Balbino
author
Malumbres Martinez, Marcos
author
Martin-Cofreces, Noa B.
author
Sanchez-Madrid, Francisco
funder
Ministerio de Economía y Competitividad (España)
funder
Comunidad de Madrid (España)
funder
Unión Europea. Comisión Europea
funder
Fundación ProCNIC
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
2017-10-30T13:32:26Z
2017-10-30T13:32:26Z
2016
Nat Commun. 2016; 7:11389
10.1038/ncomms11389
2041-1723
Nature Communications
27091106
http://hdl.handle.net/20.500.12105/5241
Aurora A is a serine/threonine kinase that contributes to the progression of mitosis by inducing microtubule nucleation. Here we have identified an unexpected role for Aurora A kinase in antigen-driven T-cell activation. We find that Aurora A is phosphorylated at the immunological synapse (IS) during TCR-driven cell contact. Inhibition of Aurora A with pharmacological agents or genetic deletion in human or mouse T cells severely disrupts the dynamics of microtubules and CD3z-bearing vesicles at the IS. The absence of Aurora A activity also impairs the activation of early signalling molecules downstream of the TCR and the expression of IL-2, CD25 and CD69. Aurora A inhibition causes delocalized clustering of Lck at the IS and decreases phosphorylation levels of tyrosine kinase Lck, thus indicating Aurora A is required for maintaining Lck active. These findings implicate Aurora A in the propagation of the TCR activation signal.
eng
IMMUNOLOGICAL SYNAPSE
IMMUNE SYNAPSE
TIRF MICROSCOPY
CENTROSOME MATURATION
ANTIGEN RECEPTOR
TYROSINE KINASE
A KINASE
IN-VIVO
LCK
ACTIN
Aurora A drives early signalling and vesicle dynamics during T-cell activation
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