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oai:repisalud.isciii.es:20.500.12105/52412025-02-27T11:24:52Zcom_20.500.12105_19604com_20.500.12105_2051com_20.500.12105_2173col_20.500.12105_19605col_20.500.12105_19597col_20.500.12105_19607

00925njm 22002777a 4500 dc Blas-Rus, Noelia author Bustos-Moran, Eugenio author Perez de Castro, Ignacio author de Carcer Diez, Guillermo author Borroto, Aldo author Camafeita, Emilio author Jorge, Inmaculada author Vazquez, Jesus author Alarcón, Balbino author Malumbres Martinez, Marcos author Martin-Cofreces, Noa B. author Sanchez-Madrid, Francisco author 2016 Aurora A is a serine/threonine kinase that contributes to the progression of mitosis by inducing microtubule nucleation. Here we have identified an unexpected role for Aurora A kinase in antigen-driven T-cell activation. We find that Aurora A is phosphorylated at the immunological synapse (IS) during TCR-driven cell contact. Inhibition of Aurora A with pharmacological agents or genetic deletion in human or mouse T cells severely disrupts the dynamics of microtubules and CD3z-bearing vesicles at the IS. The absence of Aurora A activity also impairs the activation of early signalling molecules downstream of the TCR and the expression of IL-2, CD25 and CD69. Aurora A inhibition causes delocalized clustering of Lck at the IS and decreases phosphorylation levels of tyrosine kinase Lck, thus indicating Aurora A is required for maintaining Lck active. These findings implicate Aurora A in the propagation of the TCR activation signal. Nat Commun. 2016; 7:11389 10.1038/ncomms11389 2041-1723 Nature Communications 27091106 http://hdl.handle.net/20.500.12105/5241 IMMUNOLOGICAL SYNAPSE IMMUNE SYNAPSE TIRF MICROSCOPY CENTROSOME MATURATION ANTIGEN RECEPTOR TYROSINE KINASE A KINASE IN-VIVO LCK ACTIN Aurora A drives early signalling and vesicle dynamics during T-cell activation